Detection of circulating fetal cells utilizing automated microscopy: potential for noninvasive prenatal diagnosis of chromosomal aneuploidies

Seppo, A; Frisova, V; Ichetovkin, I; Kim, YM; Evan, MI; Antsaklis, A; Nicolaides, KH; Tafas, R; Tsipouras, P; Kilpatrick, MW

dc.contributor.author	Seppo, A	en
dc.contributor.author	Frisova, V	en
dc.contributor.author	Ichetovkin, I	en
dc.contributor.author	Kim, YM	en
dc.contributor.author	Evan, MI	en
dc.contributor.author	Antsaklis, A	en
dc.contributor.author	Nicolaides, KH	en
dc.contributor.author	Tafas, R	en
dc.contributor.author	Tsipouras, P	en
dc.contributor.author	Kilpatrick, MW	en
dc.date.accessioned	2014-03-01T01:57:23Z
dc.date.available	2014-03-01T01:57:23Z
dc.date.issued	2008	en
dc.identifier.issn	0197-3851	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/28408
dc.subject	circulating fetal cells	en
dc.subject	aneuploidy detection	en
dc.subject	FISH	en
dc.subject	genetic testing	en
dc.subject	automated microscopy	en
dc.subject.classification	Genetics & Heredity	en
dc.subject.classification	Obstetrics & Gynecology	en
dc.subject.other	AMNIOTIC-FLUID CELLS	en
dc.subject.other	MATERNAL BLOOD	en
dc.subject.other	DOWNS-SYNDROME	en
dc.subject.other	1ST-TRIMESTER	en
dc.subject.other	ERYTHROBLASTS	en
dc.subject.other	PREGNANCIES	en
dc.subject.other	SIGNALS	en
dc.subject.other	SYSTEM	en
dc.subject.other	RISK	en
dc.subject.other	BETA	en
dc.title	Detection of circulating fetal cells utilizing automated microscopy: potential for noninvasive prenatal diagnosis of chromosomal aneuploidies	en
heal.type	journalArticle	en
heal.language	English	en
heal.publicationDate	2008	en
heal.abstract	Objective As fetal cells can be indisputably identified through detection of Y FISH signals, we utilized all automated microscopy system developed to identify and enumerate cells bearing X and Y FISH signals. We further investipted the potential offetal licnioglobin expression as a gender independent marker for automated identification of fetal cells. Method For FISH-based scanning, verified fetal cells were identified based oil the presence of a single X-signal and individual signals for each of the two Y FISH probes. For cell identification based on fetal hemoglobin expression, Putative fetal cells were verified based on the presence of signals for anti-gamma or anti-E globin antibody, and FISH signals for the X- and Y-chromosomes. Results Fetal cells were identified, by FISH-based scanning, in 28 of the 29 maternal samples from pregnancies with male fetuses. Simple density gradient centrifugation achieved a 3- to 5-fold increase in the number of fetal cells detected. Conclusion Automated microscopy identified fetal cells in both first and second trimester maternal blood samples. Although We Were unable to detect fetal erythroblasts ill numbers sufficient for clinical diagnosis, C the ability to reliably detect fetal cells by FISH-based scanning opens the possibility for prenatal detection of chromosomal aberrations utilizing circulating fetal cells. Copyright (c) 2008 John Wiley & Sons. Ltd.	en
heal.publisher	JOHN WILEY & SONS LTD	en
heal.journalName	PRENATAL DIAGNOSIS	en
dc.identifier.isi	ISI:000259720000006	en
dc.identifier.volume	28	en
dc.identifier.issue	9	en
dc.identifier.spage	815	en
dc.identifier.epage	821	en