Prevalence and characterization of class 1 integrons in escherichia coli of poultry and human origin

Vasilakopoulou, A; Psichogiou, M; Tzouvelekis, L; Tassios, PT; Kosmidis, C; Petrikkos, G; Roma, ES; Charvalos, E; Passiotou, M; Avlami, A; Daikos, GL

dc.contributor.author	Vasilakopoulou, A	en
dc.contributor.author	Psichogiou, M	en
dc.contributor.author	Tzouvelekis, L	en
dc.contributor.author	Tassios, PT	en
dc.contributor.author	Kosmidis, C	en
dc.contributor.author	Petrikkos, G	en
dc.contributor.author	Roma, ES	en
dc.contributor.author	Charvalos, E	en
dc.contributor.author	Passiotou, M	en
dc.contributor.author	Avlami, A	en
dc.contributor.author	Daikos, GL	en
dc.date.accessioned	2014-03-01T01:58:48Z
dc.date.available	2014-03-01T01:58:48Z
dc.date.issued	2009	en
dc.identifier.issn	15353141	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/28733
dc.subject.other	amikacin	en
dc.subject.other	ampicillin	en
dc.subject.other	antibiotic agent	en
dc.subject.other	antiinfective agent	en
dc.subject.other	aztreonam	en
dc.subject.other	cefalotin	en
dc.subject.other	cefepime	en
dc.subject.other	cefotaxime	en
dc.subject.other	cefoxitin	en
dc.subject.other	cefuroxime	en
dc.subject.other	chloramphenicol	en
dc.subject.other	ciprofloxacin	en
dc.subject.other	cotrimoxazole	en
dc.subject.other	genomic DNA	en
dc.subject.other	gentamicin	en
dc.subject.other	imipenem	en
dc.subject.other	nalidixic acid	en
dc.subject.other	piperacillin plus tazobactam	en
dc.subject.other	streptomycin	en
dc.subject.other	tetracycline	en
dc.subject.other	tobramycin	en
dc.subject.other	animal tissue	en
dc.subject.other	article	en
dc.subject.other	controlled study	en
dc.subject.other	disk diffusion	en
dc.subject.other	DNA sequence	en
dc.subject.other	drug sensitivity	en
dc.subject.other	Escherichia coli	en
dc.subject.other	gene cassette	en
dc.subject.other	heterozygote	en
dc.subject.other	hospital patient	en
dc.subject.other	human	en
dc.subject.other	integron	en
dc.subject.other	multidrug resistance	en
dc.subject.other	nonhuman	en
dc.subject.other	outpatient	en
dc.subject.other	phenotype	en
dc.subject.other	phylogenetic tree	en
dc.subject.other	polymerase chain reaction	en
dc.subject.other	poultry	en
dc.subject.other	prevalence	en
dc.subject.other	priority journal	en
dc.subject.other	pulsed field gel electrophoresis	en
dc.subject.other	reservoir	en
dc.subject.other	Animals	en
dc.subject.other	Blood	en
dc.subject.other	Disk Diffusion Antimicrobial Tests	en
dc.subject.other	DNA, Bacterial	en
dc.subject.other	Drug Resistance, Microbial	en
dc.subject.other	Drug Resistance, Multiple, Bacterial	en
dc.subject.other	Escherichia coli	en
dc.subject.other	Foodborne Diseases	en
dc.subject.other	Gastrointestinal Tract	en
dc.subject.other	Greece	en
dc.subject.other	Humans	en
dc.subject.other	Inpatients	en
dc.subject.other	Integrons	en
dc.subject.other	Outpatients	en
dc.subject.other	Phenotype	en
dc.subject.other	Polymerase Chain Reaction	en
dc.subject.other	Poultry	en
dc.subject.other	Sputum	en
dc.subject.other	Suppuration	en
dc.subject.other	Urine	en
dc.subject.other	Escherichia coli	en
dc.title	Prevalence and characterization of class 1 integrons in escherichia coli of poultry and human origin	en
heal.type	journalArticle	en
heal.identifier.primary	10.1089/fpd.2009.0281	en
heal.identifier.secondary	http://dx.doi.org/10.1089/fpd.2009.0281	en
heal.publicationDate	2009	en
heal.abstract	A prospective study was conducted to determine the prevalence and the gene-cassette content of class 1 integrons in Escherichia coli of poultry and human origin. A total of 235 E. coli isolates were examined; 65 were derived from farm poultry, 80 from hospitalized, and 90 from nonhospitalized patients. Susceptibilities to a range of antimicrobial agents were determined by disk diffusion. Int1-specific polymerase chain reaction, conserved-segment polymerase chain reaction, and DNA sequencing were used to determine the presence, length, and content of integrons. The relatedness among the isolates was examined by pulsed-field gel electrophoresis of XbaI digests of genomic DNA. The integron carriage rate for poultry isolates was 49.2%, whereas the carriage rate for hospital isolates was 26.2% and for community 11.1%. Multidrug resistance (resistance to three or more classes of antibiotics) phenotypes were observed in 96.8% of the integron-positive isolates, whereas only 34.9% of nonintegron-carrying organisms were multidrug resistant (p<0.001). Seven integron types ranging in size from 663 to 2674bp were identified; six types were observed in poultry isolates, five in hospital, and three in community isolates. Each integron type carried a distinct gene-cassette combination. The most prevalent gene cassettes belonged to the aad and dfr families. Identical integrons were detected in E. coli of human and poultry origin. A large reservoir of integrons exists in E. coli of poultry origin. The horizontal transfer of class 1 integrons among bacteria of poultry and human origins may contribute in the dissemination of antimicrobial resistance. © 2009, Mary Ann Liebert, Inc.	en
heal.journalName	Foodborne Pathogens and Disease	en
dc.identifier.doi	10.1089/fpd.2009.0281	en
dc.identifier.volume	6	en
dc.identifier.issue	10	en
dc.identifier.spage	1211	en
dc.identifier.epage	1218	en