Mucinous Colorectal Adenocarcinoma with Signet-Ring Cells: Immunohistochemical and Ultrastructural Study

Seretis, E; Konstantinidou, A; Arnogiannakis, N; Xinopoulos, D; Voloudakis-Baltatzis, IE

dc.contributor.author	Seretis, E	en
dc.contributor.author	Konstantinidou, A	en
dc.contributor.author	Arnogiannakis, N	en
dc.contributor.author	Xinopoulos, D	en
dc.contributor.author	Voloudakis-Baltatzis, IE	en
dc.date.accessioned	2014-03-01T02:00:30Z
dc.date.available	2014-03-01T02:00:30Z
dc.date.issued	2010	en
dc.identifier.issn	0191-3123	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/29109
dc.subject	colorectal	en
dc.subject	electron microscopy	en
dc.subject	immunogold method	en
dc.subject	mucinous adenocarcinoma	en
dc.subject	signet-ring cell	en
dc.subject.classification	Microscopy	en
dc.subject.classification	Pathology	en
dc.subject.other	MULTIVARIATE-ANALYSIS	en
dc.subject.other	CARCINOMA	en
dc.subject.other	COLON	en
dc.subject.other	RECTUM	en
dc.subject.other	SURVIVAL	en
dc.subject.other	SOMATOSTATIN	en
dc.subject.other	SEROTONIN	en
dc.subject.other	COMPONENT	en
dc.subject.other	FEATURES	en
dc.subject.other	BOMBESIN	en
dc.title	Mucinous Colorectal Adenocarcinoma with Signet-Ring Cells: Immunohistochemical and Ultrastructural Study	en
heal.type	journalArticle	en
heal.language	English	en
heal.publicationDate	2010	en
heal.abstract	A primary mucinous colorectal adenocarcinoma tissue with signet-ring cells, as revealed after histological evaluation, was examined ultrastructurally. The authors also analyzed the immunohistochemical data of the tissue for serotonin, vasoactive intestinal polypeptide (VIP), bombesin, somatostatin, and glucagon, using the peroxidase anti-peroxidase (PAP) method and the immunogold labeling method for light and electron microscope, respectively. Electron microscopically mucinous adenocarcinoma was characterized by the formation of small lumen. Adenocarcinoma cells were full of mucous granules of varying electron density, providing a good environment for the tumor cells to grow. They also exhibited a significant loss of microvilli and intracytoplasmic junctions, which could allow the cells to disseminate. Signet-ring cells were located in the basal site of the ducts or in the lamina propria and appeared neoplastic, with mucin accumulation intracellularly and an eccentric crescent-shaped nucleus. The cytoplasmic organelles were decreased and at the periphery of the cell. The PAP method demonstrated that these cells were strongly positive for bombesin and also positive for vasointestinal polypeptide (VIP). The immunogold method detected bombesin immunoreactivity in the vacuoles as well as in other cytoplasmic membranes, whereas VIP was localized mainly in the plasma membrane. The location of signet-ring cells combined with the immunoreactivity for bombesin and VIP indicated that signet-ring cells were of neuroendocrine origin and probably dedifferentiated enterochromaffin-like endocrine cells. These findings have implications for understanding the biological behavior of these composite malignant tumors and could help in the knowledge of the origin of signet-ring cells.	en
heal.publisher	INFORMA HEALTHCARE	en
heal.journalName	ULTRASTRUCTURAL PATHOLOGY	en
dc.identifier.isi	ISI:000284119600005	en
dc.identifier.volume	34	en
dc.identifier.issue	6	en
dc.identifier.spage	337	en
dc.identifier.epage	343	en