Associations of xenobiotic-metabolizing enzyme genotypes PON1Q192R, PON1L55M and CYP1A1*2A MspI with pathological symptoms of a rural population in south Greece

Tsatsakis, AM; Androutsopoulos, VP; Zafiropoulos, A; Babatsikou, F; Alegakis, T; Dialyna, I; Tzatzarakis, M; Koutis, C

dc.contributor.author	Tsatsakis, AM	en
dc.contributor.author	Androutsopoulos, VP	en
dc.contributor.author	Zafiropoulos, A	en
dc.contributor.author	Babatsikou, F	en
dc.contributor.author	Alegakis, T	en
dc.contributor.author	Dialyna, I	en
dc.contributor.author	Tzatzarakis, M	en
dc.contributor.author	Koutis, C	en
dc.date.accessioned	2014-03-01T02:01:45Z
dc.date.available	2014-03-01T02:01:45Z
dc.date.issued	2011	en
dc.identifier.issn	00498254	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/29245
dc.subject	Associations	en
dc.subject	Cytochrome P450 CYP1A1	en
dc.subject	Organochlorine pesticides	en
dc.subject	Organophosphate pesticides	en
dc.subject	Paraoxonase 1	en
dc.subject	Polymorphisms	en
dc.subject.other	aryldialkylphosphatase 1	en
dc.subject.other	chlorphenotane	en
dc.subject.other	cholesterol	en
dc.subject.other	cytochrome P450 1A1	en
dc.subject.other	lindane	en
dc.subject.other	organochlorine pesticide	en
dc.subject.other	organophosphate pesticide	en
dc.subject.other	adult	en
dc.subject.other	aged	en
dc.subject.other	agricultural worker	en
dc.subject.other	allele	en
dc.subject.other	article	en
dc.subject.other	blood analysis	en
dc.subject.other	chemical analysis	en
dc.subject.other	cholesterol blood level	en
dc.subject.other	cohort analysis	en
dc.subject.other	controlled study	en
dc.subject.other	demography	en
dc.subject.other	diabetes mellitus	en
dc.subject.other	disease activity	en
dc.subject.other	environmental exposure	en
dc.subject.other	enzyme activity	en
dc.subject.other	enzyme blood level	en
dc.subject.other	female	en
dc.subject.other	gene frequency	en
dc.subject.other	genetic association	en
dc.subject.other	genetic polymorphism	en
dc.subject.other	genotype	en
dc.subject.other	genotype environment interaction	en
dc.subject.other	Greece	en
dc.subject.other	hair analysis	en
dc.subject.other	hepatitis A	en
dc.subject.other	heterozygote	en
dc.subject.other	homozygote	en
dc.subject.other	human	en
dc.subject.other	hypertension	en
dc.subject.other	major clinical study	en
dc.subject.other	male	en
dc.subject.other	mass fragmentography	en
dc.subject.other	medical examination	en
dc.subject.other	medical record review	en
dc.subject.other	occupational exposure	en
dc.subject.other	polymerase chain reaction	en
dc.subject.other	prostate hypertrophy	en
dc.subject.other	questionnaire	en
dc.subject.other	restriction fragment length polymorphism	en
dc.subject.other	rural population	en
dc.subject.other	Adult	en
dc.subject.other	Aged	en
dc.subject.other	Aryldialkylphosphatase	en
dc.subject.other	Cohort Studies	en
dc.subject.other	Cytochrome P-450 CYP1A1	en
dc.subject.other	Diabetes Mellitus	en
dc.subject.other	Environmental Exposure	en
dc.subject.other	Environmental Pollutants	en
dc.subject.other	Female	en
dc.subject.other	Gene Frequency	en
dc.subject.other	Genotype	en
dc.subject.other	Greece	en
dc.subject.other	Hepatitis A	en
dc.subject.other	Humans	en
dc.subject.other	Hydrocarbons, Chlorinated	en
dc.subject.other	Hypertension	en
dc.subject.other	Male	en
dc.subject.other	Metabolic Detoxication, Drug	en
dc.subject.other	Middle Aged	en
dc.subject.other	Pesticides	en
dc.subject.other	Phosphoric Acid Esters	en
dc.subject.other	Polymorphism, Genetic	en
dc.subject.other	Prevalence	en
dc.subject.other	Prostatic Hyperplasia	en
dc.subject.other	Xenobiotics	en
dc.title	Associations of xenobiotic-metabolizing enzyme genotypes PON1Q192R, PON1L55M and CYP1A1*2A MspI with pathological symptoms of a rural population in south Greece	en
heal.type	journalArticle	en
heal.identifier.primary	10.3109/00498254.2011.590545	en
heal.identifier.secondary	http://dx.doi.org/10.3109/00498254.2011.590545	en
heal.publicationDate	2011	en
heal.abstract	Paraoxonases and cytochromes P450 constitute two major classes of xenobiotic-metabolizing enzymes involved in the detoxification of pesticide chemicals. In this study, we examined the distribution of two common genetic polymorphisms of the paraoxonase 1 gene and one common polymorphism of the CYP1A1 gene, in relation to pathological diseases occurring in a rural population. Blood and hair samples were collected from 220 participants of an agricultural cohort in the south of Greece for genotype and pesticide analysis. Demographic information and disease status of the participants was obtained by questionnaire, medical examination and medical record. Organochlorine pesticides and metabolites (DDTs, HCHs) were extracted from hair and analyzed using gas chromatography combined with mass spectrometry techniques. Our results indicate exposure of the rural population of Amaliada to organophosphate and past exposure to organochlorine pesticides. Genotypic analysis of PON1Q192R, PON1L55M and CYP1A1*2A MspI polymorphisms was performed using PCR-RFLP. The PON1 192R and 55M alleles absence was significantly associated with hypertension (OR: 2.59; 95% CI: 1.10-6.09) and hepatitis (OR: 21.43; 95% CI: 2.53-181.50), respectively, as indicated from backward logistic regression. Although the presence of PON1 192R allele significantly affected the occurrence of prostate hyperplasia (OR: 0.35; 95% CI: 0.03-0.40), no associations were obtained between the paraoxonase serum activity or the CYP1A1 genotype and the disease status. © 2011 Informa UK, Ltd.	en
heal.journalName	Xenobiotica	en
dc.identifier.doi	10.3109/00498254.2011.590545	en
dc.identifier.volume	41	en
dc.identifier.issue	10	en
dc.identifier.spage	914	en
dc.identifier.epage	925	en