Characterization of Enterobius vermicularis in a human population, employing a molecular-based method from adhesive tape samples

Piperaki, ET; Spanakos, G; Patsantara, G; Vassalou, E; Vakalis, N; Tsakris, A

dc.contributor.author	Piperaki, ET	en
dc.contributor.author	Spanakos, G	en
dc.contributor.author	Patsantara, G	en
dc.contributor.author	Vassalou, E	en
dc.contributor.author	Vakalis, N	en
dc.contributor.author	Tsakris, A	en
dc.date.accessioned	2014-03-01T02:04:25Z
dc.date.available	2014-03-01T02:04:25Z
dc.date.issued	2011	en
dc.identifier.issn	0890-8508	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/29431
dc.subject	Enterobius vermicularis	en
dc.subject	Genotyping	en
dc.subject	Human	en
dc.subject	Adhesive tape	en
dc.subject.classification	Biochemical Research Methods	en
dc.subject.classification	Biochemistry & Molecular Biology	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.classification	Cell Biology	en
dc.subject.other	C-OXIDASE SUBUNIT-1	en
dc.subject.other	CAPTIVE CHIMPANZEES	en
dc.subject.other	HUMAN COPROLITES	en
dc.subject.other	DNA-SEQUENCES	en
dc.subject.other	ANCIENT DNA	en
dc.subject.other	GENE	en
dc.subject.other	PINWORM	en
dc.subject.other	NEMATODES	en
dc.subject.other	PARASITES	en
dc.subject.other	SPACER	en
dc.title	Characterization of Enterobius vermicularis in a human population, employing a molecular-based method from adhesive tape samples	en
heal.type	journalArticle	en
heal.language	English	en
heal.publicationDate	2011	en
heal.abstract	Human infection with the parasitic nematode Enterobius vermicularis occurs worldwide, particularly in children. Although its prevalence may exceed 35% in some parts of the world, molecular studies of E. vermicularis in humans are limited. The aim of the present study was to investigate the genetic variation within E. vermicularis in a human population. For this purpose, 77 adhesive tape samples taken from Greek children infested with E. vermicularis were tested. New primers were designed to amplify a segment of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene of E. vermicularis from adhesive tape samples. Thirty-six amplicons were sequenced and eleven different haplotypes were identified. All sequences clustered within the type previously characterized (type B), only reported to date from captive chimpanzees. To the best of our knowledge, this is the first study of E. vermicularis genotypes from a human population. (C) 2011 Elsevier Ltd. All rights reserved.	en
heal.publisher	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD	en
heal.journalName	MOLECULAR AND CELLULAR PROBES	en
dc.identifier.isi	ISI:000290609100008	en
dc.identifier.volume	25	en
dc.identifier.issue	2-3	en
dc.identifier.spage	121	en
dc.identifier.epage	125	en