Alcohol production from sterilized and non-sterilized molasses by Saccharomyces cerevisiae immobilized on brewer's spent grains in two types of continuous bioreactor systems

Kopsahelis, N; Bosnea, L; Bekatorou, A; Tzia, C; Kanellaki, M

dc.contributor.author	Kopsahelis, N	en
dc.contributor.author	Bosnea, L	en
dc.contributor.author	Bekatorou, A	en
dc.contributor.author	Tzia, C	en
dc.contributor.author	Kanellaki, M	en
dc.date.accessioned	2014-03-01T02:07:36Z
dc.date.available	2014-03-01T02:07:36Z
dc.date.issued	2012	en
dc.identifier.issn	09619534	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/29590
dc.subject	Brewer's spent grains	en
dc.subject	Continuous alcoholic fermentation	en
dc.subject	Ethanol	en
dc.subject	Immobilized yeast cells	en
dc.subject	Non-sterilized molasses	en
dc.subject.other	Alcoholic fermentation	en
dc.subject.other	Brewer's spent grain	en
dc.subject.other	Continuous bioreactors	en
dc.subject.other	Continuous operation	en
dc.subject.other	Cost effective	en
dc.subject.other	Ethanol concentrations	en
dc.subject.other	Fermentation efficiency	en
dc.subject.other	Fixed bed	en
dc.subject.other	Immobilized yeasts	en
dc.subject.other	Operational stability	en
dc.subject.other	Packed bed reactor	en
dc.subject.other	SIMPLE method	en
dc.subject.other	Sterilization process	en
dc.subject.other	Temperature range	en
dc.subject.other	Bioconversion	en
dc.subject.other	Bioreactors	en
dc.subject.other	Ethanol	en
dc.subject.other	Fermentation	en
dc.subject.other	Packed beds	en
dc.subject.other	Yeast	en
dc.subject.other	alcohol	en
dc.subject.other	bioreactor	en
dc.subject.other	brewing industry	en
dc.subject.other	concentration (composition)	en
dc.subject.other	fermentation	en
dc.subject.other	immobilization	en
dc.subject.other	industrial production	en
dc.subject.other	molasse	en
dc.subject.other	yeast	en
dc.subject.other	Saccharomyces cerevisiae	en
dc.title	Alcohol production from sterilized and non-sterilized molasses by Saccharomyces cerevisiae immobilized on brewer's spent grains in two types of continuous bioreactor systems	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.biombioe.2012.05.015	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.biombioe.2012.05.015	en
heal.publicationDate	2012	en
heal.abstract	In this work an integrated cost effective system for continuous alcoholic fermentation of a cheap raw material (molasses) is described, involving yeast immobilized by a simple method on brewer's spent grains, able to ferment in the temperature range 30-40 °C, and two types of bioreactors, a Multistage Fixed Bed Tower (MFBT) and a Packed Bed reactor (PB). The MFBT bioreactor gave better results regarding ethanol concentration, productivity and conversion. Furthermore, the use of sterilized and non-sterilized molasses, fed in two similar MFBT bioreactors, showed that ethanol concentration (kg m-3) was significantly (p < 0.05) affected by the sterilization process as well as temperature alteration. Higher ethanol concentrations were obtained in the case of non-sterilized molasses (47.4-50.6 kg m-3at 35 °C and 44.2-48.2 kg m-3 at 40 °C), compared to sterilized molasses, where ethanol concentration ranged from 35.6 to 46.6 kg m-3 at 35 °C and 30.8-44.2 kg m-3at 40 °C. During 32 days of continuous operation using non-sterilized molasses no contamination was observed. Industrialization of the proposed system seems to have a potential, mainly due to its high fermentation efficiency and the obtained high operational stability. © 2012 Elsevier Ltd.	en
heal.journalName	Biomass and Bioenergy	en
dc.identifier.doi	10.1016/j.biombioe.2012.05.015	en
dc.identifier.volume	45	en
dc.identifier.spage	87	en
dc.identifier.epage	94	en