Bioconversion of dilute-acid pretreated sorghum bagasse to ethanol by Neurospora crassa

Dogaris, I; Gkounta, O; Mamma, D; Kekos, D

dc.contributor.author	Dogaris, I	en
dc.contributor.author	Gkounta, O	en
dc.contributor.author	Mamma, D	en
dc.contributor.author	Kekos, D	en
dc.date.accessioned	2014-03-01T02:08:01Z
dc.date.available	2014-03-01T02:08:01Z
dc.date.issued	2012	en
dc.identifier.issn	01757598	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/29626
dc.subject	Bioconversion	en
dc.subject	Cellulase	en
dc.subject	Ethanol	en
dc.subject	Filamentous fungi	en
dc.subject	Sorghum bagasse	en
dc.subject	Yeast	en
dc.subject.other	Bio-ethanol production	en
dc.subject.other	Cellulase	en
dc.subject.other	Cellulase activity	en
dc.subject.other	Cofermentation	en
dc.subject.other	Cost-efficient	en
dc.subject.other	Depolymerase	en
dc.subject.other	Dilute-acid	en
dc.subject.other	Energy yields	en
dc.subject.other	Ethanol production	en
dc.subject.other	Filamentous fungi	en
dc.subject.other	Glucosidase	en
dc.subject.other	Hemicellulose hydrolysis	en
dc.subject.other	Hemicellulose sugars	en
dc.subject.other	High-solids	en
dc.subject.other	In-situ	en
dc.subject.other	Mixed cultures	en
dc.subject.other	Neurospora crassa	en
dc.subject.other	Pre-Treatment	en
dc.subject.other	S.cerevisiae	en
dc.subject.other	Solid residues	en
dc.subject.other	Sweet sorghum	en
dc.subject.other	Theoretical yield	en
dc.subject.other	Bagasse	en
dc.subject.other	Bioconversion	en
dc.subject.other	Cellulose	en
dc.subject.other	Ethanol	en
dc.subject.other	Sugars	en
dc.subject.other	Sulfuric acid	en
dc.subject.other	Yeast	en
dc.subject.other	Bioethanol	en
dc.subject.other	alcohol	en
dc.subject.other	bagasse	en
dc.subject.other	beta glucosidase	en
dc.subject.other	bioethanol	en
dc.subject.other	biofuel	en
dc.subject.other	cellulase	en
dc.subject.other	cellulose	en
dc.subject.other	hemicellulose	en
dc.subject.other	sulfuric acid	en
dc.subject.other	unclassified drug	en
dc.subject.other	biofuel	en
dc.subject.other	cellulose	en
dc.subject.other	crop yield	en
dc.subject.other	energy crop	en
dc.subject.other	enzyme activity	en
dc.subject.other	ethanol	en
dc.subject.other	fermentation	en
dc.subject.other	hydrolysis	en
dc.subject.other	lignin	en
dc.subject.other	sorghum	en
dc.subject.other	sugar	en
dc.subject.other	yeast	en
dc.subject.other	alternative energy	en
dc.subject.other	article	en
dc.subject.other	biofuel production	en
dc.subject.other	biotransformation	en
dc.subject.other	decomposition	en
dc.subject.other	enzyme activity	en
dc.subject.other	enzyme substrate	en
dc.subject.other	fungus	en
dc.subject.other	fungus culture	en
dc.subject.other	Neurospora crassa	en
dc.subject.other	nonhuman	en
dc.subject.other	Saccharomyces cerevisiae	en
dc.subject.other	sorghum	en
dc.subject.other	yeast	en
dc.subject.other	Bagasse	en
dc.subject.other	Cellulase	en
dc.subject.other	Cellulose	en
dc.subject.other	Ethanol	en
dc.subject.other	Fungi	en
dc.subject.other	Sorghum	en
dc.subject.other	Sugars	en
dc.subject.other	Sulfuric Acid	en
dc.subject.other	Yeasts	en
dc.subject.other	Fungi	en
dc.subject.other	Neurospora crassa	en
dc.subject.other	Saccharomyces cerevisiae	en
dc.title	Bioconversion of dilute-acid pretreated sorghum bagasse to ethanol by Neurospora crassa	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s00253-012-4113-1	en
heal.identifier.secondary	http://dx.doi.org/10.1007/s00253-012-4113-1	en
heal.publicationDate	2012	en
heal.abstract	Bioethanol production from sweet sorghum bagasse (SB), the lignocellulosic solid residue obtained after extraction of sugars from sorghum stalks, can further improve the energy yield of the crop. The aim of the present work was to evaluate a cost-efficient bioconversion of SB to ethanol at high solids loadings (16 % at pretreatment and 8 % at fermentation), low cellulase activities (1-7 FPU/g SB) and cofermentation of hexoses and pentoses. The fungus Neurospora crassa DSM 1129 was used, which exhibits both depolymerase and co-fermentative ability, as well as mixed cultures with Saccharomyces cerevisiae 2541. A dilute-acid pretreatment (sulfuric acid 2 g/100 g SB; 210 °C; 10 min) was implemented, with high hemicellulose decomposition and low inhibitor formation. The bioconversion efficiency of N. crassa was superior to S. cerevisiae, while their mixed cultures had negative effect on ethanol production. Supplementing the in situ produced N. crassa cellulolytic system (1.0 FPU/g SB) with commercial cellulase and β-glucosidase mixture at low activity (6.0 FPU/g SB) increased ethanol production to 27.6 g/l or 84.7 % of theoretical yield (based on SB cellulose and hemicellulose sugar content). The combined dilute-acid pretreatment and bioconversion led to maximum cellulose and hemicellulose hydrolysis 73.3 % and 89.6 %, respectively. © Springer-Verlag 2012.	en
heal.journalName	Applied Microbiology and Biotechnology	en
dc.identifier.doi	10.1007/s00253-012-4113-1	en
dc.identifier.volume	95	en
dc.identifier.issue	2	en
dc.identifier.spage	541	en
dc.identifier.epage	550	en