Decrement of cellulose recalcitrance by treatment with ionic liquid (1-ethyl-3-methylimidazolium acetate) as a strategy to enhance enzymatic hydrolysis

Xiros, C; Vafiadi, C; Topakas, E; Christakopoulos, P

dc.contributor.author	Xiros, C	en
dc.contributor.author	Vafiadi, C	en
dc.contributor.author	Topakas, E	en
dc.contributor.author	Christakopoulos, P	en
dc.date.accessioned	2014-03-01T02:08:36Z
dc.date.available	2014-03-01T02:08:36Z
dc.date.issued	2012	en
dc.identifier.issn	02682575	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/29682
dc.subject	Biocatalysis	en
dc.subject	Biofuel	en
dc.subject	Biomass	en
dc.subject	Mathematical modelling	en
dc.subject	Pre-treatment	en
dc.subject.other	Biocatalysis	en
dc.subject.other	Bioconversion process	en
dc.subject.other	Bioconversion yields	en
dc.subject.other	Bioprocesses	en
dc.subject.other	Cellulose crystallinity	en
dc.subject.other	Crystalline cellulose	en
dc.subject.other	Enzymatic Degradation	en
dc.subject.other	Fusarium oxysporums	en
dc.subject.other	Glucose production	en
dc.subject.other	High conversions	en
dc.subject.other	High crystallinity	en
dc.subject.other	Hydrolysis rate	en
dc.subject.other	In-situ	en
dc.subject.other	Liquid treatment	en
dc.subject.other	Mathematical modelling	en
dc.subject.other	Molecular solvents	en
dc.subject.other	Pre-Treatment	en
dc.subject.other	Simultaneous saccharification and fermentation	en
dc.subject.other	Structural change	en
dc.subject.other	Biofuels	en
dc.subject.other	Biomass	en
dc.subject.other	Cellulose	en
dc.subject.other	Enzymatic hydrolysis	en
dc.subject.other	Glucose	en
dc.subject.other	Rate constants	en
dc.subject.other	Substrates	en
dc.subject.other	Volatile fatty acids	en
dc.subject.other	Ionic liquids	en
dc.subject.other	1 ethyl 3 methylimidazolium acetate	en
dc.subject.other	1 methylimidazole	en
dc.subject.other	cellulose	en
dc.subject.other	glucose	en
dc.subject.other	ionic liquid	en
dc.subject.other	polymer	en
dc.subject.other	unclassified drug	en
dc.subject.other	article	en
dc.subject.other	biotransformation	en
dc.subject.other	chemical reaction	en
dc.subject.other	crystallization	en
dc.subject.other	Fusarium oxysporum	en
dc.subject.other	hydrolysis	en
dc.subject.other	nonhuman	en
dc.subject.other	reaction analysis	en
dc.subject.other	solvation	en
dc.subject.other	Fusarium oxysporum	en
dc.title	Decrement of cellulose recalcitrance by treatment with ionic liquid (1-ethyl-3-methylimidazolium acetate) as a strategy to enhance enzymatic hydrolysis	en
heal.type	journalArticle	en
heal.identifier.primary	10.1002/jctb.2756	en
heal.identifier.secondary	http://dx.doi.org/10.1002/jctb.2756	en
heal.publicationDate	2012	en
heal.abstract	Background: The high crystallinity of cellulose underlies the recalcitrance that this polymer presents in enzymatic degradation. Thus, a pre-treatment step is applied in most bioconversion processes. Treatments with ionic liquids are considered an emerging pre-treatment technology, owing to their high efficiency in solvating cellulose, over molecular solvent systems. Results: Crystalline cellulose with and without ionic liquid (1-ethyl-3-methylimidazolium acetate) treatment, both commercially available, were used as substrates in enzymatic hydrolysis reactions using the earlier evaluated cellulolytic system of Fusarium oxysporum. The in situ removal of the hydrolysate during reactions enhanced the reaction rate as well as the overall glucose production. Ionic liquid treatment significantly decreased cellulose crystallinity and enhanced bioconversion yields and rates. The effects of cellulose structural changes during treatment on hydrolysis rate were investigated and the recalcitrance constants were determined. Conclusion: The study showed that ionic liquid-treated cellulose became more homogeneous and more easily degradable than the untreated cellulose, a conclusion that was expressed mathematically by the difference in the recalcitrance constants for the two substrates. It was concluded that glucose production from ionic liquid-treated cellulose could achieve very high conversion yields in consolidated bioprocesses or during simultaneous saccharification and fermentation. © 2012 Society of Chemical Industry.	en
heal.journalName	Journal of Chemical Technology and Biotechnology	en
dc.identifier.doi	10.1002/jctb.2756	en
dc.identifier.volume	87	en
dc.identifier.issue	5	en
dc.identifier.spage	629	en
dc.identifier.epage	634	en