Effect of low severity dilute-acid pretreatment of barley straw and decreased enzyme loading hydrolysis on the production of fermentable substrates and the release of inhibitory compounds

Panagiotopoulos, IA; Lignos, GD; Bakker, RR; Koukios, EG

dc.contributor.author	Panagiotopoulos, IA	en
dc.contributor.author	Lignos, GD	en
dc.contributor.author	Bakker, RR	en
dc.contributor.author	Koukios, EG	en
dc.date.accessioned	2014-03-01T02:08:42Z
dc.date.available	2014-03-01T02:08:42Z
dc.date.issued	2012	en
dc.identifier.issn	09596526	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/29714
dc.subject	Barley straw	en
dc.subject	Dilute-acid pretreatment	en
dc.subject	Enzymatic hydrolysis	en
dc.subject	Fermentable substrate	en
dc.subject	Inhibitors	en
dc.subject	Sugar yield	en
dc.subject.other	5 hydroxymethyl furfurals	en
dc.subject.other	Acid loading	en
dc.subject.other	Dilute-acid	en
dc.subject.other	Enzyme loading	en
dc.subject.other	Filter paper units	en
dc.subject.other	Inhibitory compounds	en
dc.subject.other	Levulinic acid	en
dc.subject.other	Pre-Treatment	en
dc.subject.other	Pre-treatments	en
dc.subject.other	Sugar yield	en
dc.subject.other	Toxic levels	en
dc.subject.other	Acetic acid	en
dc.subject.other	Aldehydes	en
dc.subject.other	Corrosion inhibitors	en
dc.subject.other	Enzymatic hydrolysis	en
dc.subject.other	Experiments	en
dc.subject.other	Formic acid	en
dc.subject.other	Furfural	en
dc.subject.other	Organic acids	en
dc.subject.other	pH	en
dc.subject.other	Substrates	en
dc.subject.other	Sugars	en
dc.subject.other	Loading	en
dc.subject.other	Hordeum	en
dc.title	Effect of low severity dilute-acid pretreatment of barley straw and decreased enzyme loading hydrolysis on the production of fermentable substrates and the release of inhibitory compounds	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.jclepro.2012.03.019	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.jclepro.2012.03.019	en
heal.publicationDate	2012	en
heal.abstract	The objective of this work was to investigate the feasibility of combining low severity dilute-acid pretreatment of barley straw and decreased enzyme loading hydrolysis for the high production of fermentable substrates and the low release of inhibitory compounds. For most of the pretreatments at 160 and 180 °C, the sugar production with 15 FPU (filter paper unit)/g straw was equally high compared to higher enzyme loadings. For the pretreatments at 170°C an enzyme loading higher than 15 FPU/g straw was necessary to achieve a carbohydrate conversion of 50% or higher. The effect of acid loading on sugar production was discernible only in the experiments with 15 FPU/g straw or higher. The concentration of 5-hydroxymethylfurfural (HMF), levulinic acid and formic acid was kept below 0.7, 0.6 and 0.8 g L-1, respectively, with all experiments. The release of acetic acid and furfural reached toxic levels with experiments at 170°C and experiments at 180°C, respectively. Decreasing the enzyme loading did not have a major effect on the release of HMF, furfural and formic acid but resulted in decreased release of acetic acid and levulinic acid at 170 and 180°C. © 2012 Elsevier Ltd. All rights reserved.	en
heal.journalName	Journal of Cleaner Production	en
dc.identifier.doi	10.1016/j.jclepro.2012.03.019	en
dc.identifier.volume	32	en
dc.identifier.spage	45	en
dc.identifier.epage	51	en