Protein and water dynamics in bovine serum albumin-water mixtures over wide ranges of composition

Panagopoulou, A; Kyritsis, A; Shinyashiki, N; Pissis, P

dc.contributor.author	Panagopoulou, A	en
dc.contributor.author	Kyritsis, A	en
dc.contributor.author	Shinyashiki, N	en
dc.contributor.author	Pissis, P	en
dc.date.accessioned	2014-03-01T02:12:04Z
dc.date.available	2014-03-01T02:12:04Z
dc.date.issued	2012	en
dc.identifier.issn	15206106	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/30001
dc.subject.other	Arrhenius	en
dc.subject.other	Bovine serum	en
dc.subject.other	Bovine serum albumins	en
dc.subject.other	Dielectric relaxation spectroscopy	en
dc.subject.other	Dielectric response	en
dc.subject.other	Dynamic behaviors	en
dc.subject.other	Hydration levels	en
dc.subject.other	Low temperatures	en
dc.subject.other	Percolation thresholds	en
dc.subject.other	Polar groups	en
dc.subject.other	Protein surface	en
dc.subject.other	Protonic conductivities	en
dc.subject.other	Relaxation modes	en
dc.subject.other	Water dynamics	en
dc.subject.other	Water fraction	en
dc.subject.other	Water mixture	en
dc.subject.other	Water molecule	en
dc.subject.other	Body fluids	en
dc.subject.other	Dielectric relaxation	en
dc.subject.other	Glass transition	en
dc.subject.other	Hydration	en
dc.subject.other	Mammals	en
dc.subject.other	Proteins	en
dc.title	Protein and water dynamics in bovine serum albumin-water mixtures over wide ranges of composition	en
heal.type	journalArticle	en
heal.identifier.primary	10.1021/jp2105727	en
heal.identifier.secondary	http://dx.doi.org/10.1021/jp2105727	en
heal.publicationDate	2012	en
heal.abstract	Dielectric dynamic behavior of bovine serum albumin (BSA)-water mixtures over wide ranges of water fractions, from dry protein until 40 wt % in water, was studied through dielectric relaxation spectroscopy (DRS). The α relaxation associated with the glass transition of the hydrated system was identified. The evolution of the low temperature dielectric relaxation of small polar groups of the protein surface with hydration level results in the enhancement of dielectric response and the decrease of relaxation times, until a critical water fraction, which corresponds to the percolation threshold for protonic conductivity. For water fractions higher than the critical one, the position of the secondary ν relaxation of water saturates in the Arrhenius diagram, while contributions originating from water molecules in excess (uncrystallized water or ice) follow separate relaxation modes slower than the ν relaxation. © 2012 American Chemical Society.	en
heal.journalName	Journal of Physical Chemistry B	en
dc.identifier.doi	10.1021/jp2105727	en
dc.identifier.volume	116	en
dc.identifier.issue	15	en
dc.identifier.spage	4593	en
dc.identifier.epage	4602	en