Rapid Flow Injection Electrochemical Detection of Arochlor 1242 Using Stabilized Lipid Membranes with Incorporated Sheep anti-PCB Antibody

Michaloliakos, AI; Nikoleli, G-P; Siontorou, CG; Nikolelis, DP

dc.contributor.author	Michaloliakos, AI	en
dc.contributor.author	Nikoleli, G-P	en
dc.contributor.author	Siontorou, CG	en
dc.contributor.author	Nikolelis, DP	en
dc.date.accessioned	2014-03-01T02:12:05Z
dc.date.available	2014-03-01T02:12:05Z
dc.date.issued	2012	en
dc.identifier.issn	10400397	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/30007
dc.subject	Antibody regeneration	en
dc.subject	Arochlor 1242	en
dc.subject	Biosensors	en
dc.subject	Flow injection	en
dc.subject	Stabilized lipid films	en
dc.title	Rapid Flow Injection Electrochemical Detection of Arochlor 1242 Using Stabilized Lipid Membranes with Incorporated Sheep anti-PCB Antibody	en
heal.type	journalArticle	en
heal.identifier.primary	10.1002/elan.201100393	en
heal.identifier.secondary	http://dx.doi.org/10.1002/elan.201100393	en
heal.publicationDate	2012	en
heal.abstract	This work describes a novel electrochemical biosensor based on a supported polymerized lipid film with incorporated Sheep anti-PCB antibody for the rapid detection of arochlor 1242 in flowing solution streams. The antibody was incorporated into the lipid film during polymerization. Injections of antigen were made into flowing streams of a carrier electrolyte solution. Experiments were done in a stopped-flow mode using lipid mixtures containing 15% (w/w) dipalmitoylphosphatidic acid (DPPA) to provide only a single transient current signal with a magnitude related to the antigen concentration. Lipid films containing 35% DPPA were used to examine regeneration of the active sites of antibody after complex formation by washing with the carrier electrolyte solution. Repetitive cycles of injection of antigen have shown that the maximum number of cycles is about 5. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.	en
heal.journalName	Electroanalysis	en
dc.identifier.doi	10.1002/elan.201100393	en
dc.identifier.volume	24	en
dc.identifier.issue	3	en
dc.identifier.spage	495	en
dc.identifier.epage	501	en