Submerged fermentation of the edible mushroom Pleurotus ostreatus in a batch stirred tank bioreactor as a promising alternative for the effective production of bioactive metabolites

Papaspyridi, L-M; Aligiannis, N; Topakas, E; Christakopoulos, P; Skaltsounis, A-L; Fokialakis, N

dc.contributor.author	Papaspyridi, L-M	en
dc.contributor.author	Aligiannis, N	en
dc.contributor.author	Topakas, E	en
dc.contributor.author	Christakopoulos, P	en
dc.contributor.author	Skaltsounis, A-L	en
dc.contributor.author	Fokialakis, N	en
dc.date.accessioned	2014-03-01T02:14:45Z
dc.date.available	2014-03-01T02:14:45Z
dc.date.issued	2012	en
dc.identifier.issn	14203049	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/30095
dc.subject	Bioactive metabolites	en
dc.subject	Fast Centrifugal Partition Chromatography	en
dc.subject	Pleurotus ostreatus	en
dc.subject	Stirred tank bioreactor	en
dc.subject	Submerged fermentation	en
dc.subject.other	biological product	en
dc.subject.other	dichloromethane	en
dc.subject.other	fatty acid	en
dc.subject.other	methanol	en
dc.subject.other	solvent	en
dc.subject.other	article	en
dc.subject.other	batch cell culture	en
dc.subject.other	bioreactor	en
dc.subject.other	chemistry	en
dc.subject.other	fermentation	en
dc.subject.other	fractionation	en
dc.subject.other	growth, development and aging	en
dc.subject.other	isolation and purification	en
dc.subject.other	liquid chromatography	en
dc.subject.other	metabolism	en
dc.subject.other	methodology	en
dc.subject.other	Pleurotus	en
dc.subject.other	Batch Cell Culture Techniques	en
dc.subject.other	Biological Agents	en
dc.subject.other	Bioreactors	en
dc.subject.other	Chemical Fractionation	en
dc.subject.other	Chromatography, Liquid	en
dc.subject.other	Fatty Acids	en
dc.subject.other	Fermentation	en
dc.subject.other	Methanol	en
dc.subject.other	Methylene Chloride	en
dc.subject.other	Pleurotus	en
dc.subject.other	Solvents	en
dc.title	Submerged fermentation of the edible mushroom Pleurotus ostreatus in a batch stirred tank bioreactor as a promising alternative for the effective production of bioactive metabolites	en
heal.type	journalArticle	en
heal.identifier.primary	10.3390/molecules17032714	en
heal.identifier.secondary	http://dx.doi.org/10.3390/molecules17032714	en
heal.publicationDate	2012	en
heal.abstract	The aim of this study was to investigate the potential of the submerged fermentation procedure in the production of bioactive metabolites of the common edible mushroom Pleurotus ostreatus. The biomass of the mushroom strain was produced by submerged fermentation in a batch stirred tank bioreactor and extracted by solvents of increasing polarity. The dichloromethane and methanol extract were fractioned by different techniques including Adsorption Chromatography and Fast Centrifugal Partition Chromatography (FCPC). The structures of pure compounds were elucidated with 1D/2D NMR-spectroscopic analyses, and chemical correlations combined with GC/MS and LC/MS experiments. Nineteen metabolites (e.g., fatty acids, phenolic metabolites, nucleotides and alkaloids) were isolated. Beyond the production of known metabolites, we report herein the production also of trans-3,4-dihydro-3,4,8-trihydroxynapthalen-1(2H)- one, indolo-3-carboxylic acid, 3-formylpyrrole and 4-hydroxybenzoic acid, that have pharmaceutical interest and are isolated for the first time from Pleurotus strains.This work indicates the great potential of the established bioprocess for the production of P. ostreatus mycelia with enhanced metabolic profile. © 2012 by the authors.	en
heal.journalName	Molecules	en
dc.identifier.doi	10.3390/molecules17032714	en
dc.identifier.volume	17	en
dc.identifier.issue	3	en
dc.identifier.spage	2714	en
dc.identifier.epage	2724	en