Water and protein dynamics in protein-water mixtures over wide range of composition

Kyritsis, A; Panagopoulou, A; Pissis, P; Serra, RSI; Gomez Ribelles, JL; Shinyashiki, N

dc.contributor.author	Kyritsis, A	en
dc.contributor.author	Panagopoulou, A	en
dc.contributor.author	Pissis, P	en
dc.contributor.author	Serra, RSI	en
dc.contributor.author	Gomez Ribelles, JL	en
dc.contributor.author	Shinyashiki, N	en
dc.date.accessioned	2014-03-01T02:14:54Z
dc.date.available	2014-03-01T02:14:54Z
dc.date.issued	2012	en
dc.identifier.issn	10709878	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/30174
dc.subject	dielectric relaxation	en
dc.subject	glass transition	en
dc.subject	Hydrated protein	en
dc.subject	uncrystallized water	en
dc.subject.other	Bovine serum albumine	en
dc.subject.other	Dielectric relaxation spectroscopy	en
dc.subject.other	Dilute solution	en
dc.subject.other	Dry solids	en
dc.subject.other	Equilibrium sorption isotherms	en
dc.subject.other	Hydration levels	en
dc.subject.other	Percolating waters	en
dc.subject.other	Polar groups	en
dc.subject.other	Protein dynamics	en
dc.subject.other	Protein-water systems	en
dc.subject.other	Room temperature	en
dc.subject.other	Thermally stimulated depolarization currents	en
dc.subject.other	Water molecule	en
dc.subject.other	Dielectric relaxation	en
dc.subject.other	Differential scanning calorimetry	en
dc.subject.other	Dynamics	en
dc.subject.other	Glass transition	en
dc.subject.other	Hydration	en
dc.subject.other	Proteins	en
dc.title	Water and protein dynamics in protein-water mixtures over wide range of composition	en
heal.type	journalArticle	en
heal.identifier.primary	10.1109/TDEI.2012.6259997	en
heal.identifier.secondary	http://dx.doi.org/10.1109/TDEI.2012.6259997	en
heal.identifier.secondary	6259997	en
heal.publicationDate	2012	en
heal.abstract	Water and protein dynamics in two globular protein-water systems, water-lysozyme and water-BSA (bovine serum albumine), were studied by differential scanning calorimetry (DSC), dielectric relaxation spectroscopy (DRS) and thermally stimulated depolarization currents (TSDC) techniques. Water equilibrium sorption isotherms (ESI) measurements were also recorded at room temperature. The samples covered a wide range of composition, from practically dry solid pellets (2wt% of water) to dilute solutions (82wt% of water). Crystallization and melting events of water were studied by DSC and the amount of uncrystallized water was calculated. The evolution of dynamics with hydration level was followed for various dielectric relaxation processes, the emphasis being given to relaxation processes of polar groups on the surface of the proteins and of uncrystallized water molecules. A relationship between the formation of a conductive percolating water cluster and the saturation of the water v process was found. © 1994-2012 IEEE.	en
heal.journalName	IEEE Transactions on Dielectrics and Electrical Insulation	en
dc.identifier.doi	10.1109/TDEI.2012.6259997	en
dc.identifier.volume	19	en
dc.identifier.issue	4	en
dc.identifier.spage	1239	en
dc.identifier.epage	1246	en