Optimizing multienzyme production

Hatzinikolaou, DG; Macris, BJ

dc.contributor.author	Hatzinikolaou, DG	en
dc.contributor.author	Macris, BJ	en
dc.date.accessioned	2014-03-01T02:40:57Z
dc.date.available	2014-03-01T02:40:57Z
dc.date.issued	1992	en
dc.identifier.issn	0077-8923	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/30293
dc.relation.uri	http://www.scopus.com/inward/record.url?eid=2-s2.0-0027056285&partnerID=40&md5=f075d9620b4a7f1bff1e709e5741a4ee	en
dc.subject.classification	Multidisciplinary Sciences	en
dc.subject.other	carbon	en
dc.subject.other	catalase	en
dc.subject.other	glucose oxidase	en
dc.subject.other	nitrogen	en
dc.subject.other	superoxide dismutase	en
dc.subject.other	aspergillus niger	en
dc.subject.other	conference paper	en
dc.subject.other	cost	en
dc.subject.other	enzyme activity	en
dc.subject.other	enzyme assay	en
dc.subject.other	enzyme purification	en
dc.subject.other	enzyme synthesis	en
dc.subject.other	fermentation	en
dc.subject.other	fungus growth	en
dc.subject.other	model	en
dc.subject.other	nonhuman	en
dc.subject.other	priority journal	en
dc.title	Optimizing multienzyme production	en
heal.type	conferenceItem	en
heal.language	English	en
heal.publicationDate	1992	en
heal.abstract	The possibility of optimizing certain fermentation conditions towards the total unit productivity of several enzymes has been investigated. As a model system, we used a particular strain of Aspergillus niger capable of simultaneously producing significant levels of superoxide dismutase, glucose oxidase, and catalase. The fermentation parameters used in the optimization were the fermentation time, and the concentration of carbon and nitrogen source. The optimum set of fermentation parameters for maximum production of each enzyme has initially been determined. The introduction of a value factor for each enzyme unit of specific purity yields the conclusion that there is a point in the parameter space, different from that of optimum production of each separate enzyme, where a maximum exists for the total unit value of the three enzymes produced. This result suggests that multienzyme optimization in fermentations may effectively reduce the cost of the production process.	en
heal.publisher	NEW YORK ACAD SCIENCES	en
heal.journalName	Annals of the New York Academy of Sciences	en
dc.identifier.isi	ISI:A1992KJ58500083	en
dc.identifier.volume	672	en
dc.identifier.spage	595	en
dc.identifier.epage	602	en