Detecting radiation-induced DNA damage: From changes in dielectric properties to programmed cell death

Konsta, AA; Visvardis, EE; Haveles, KS; Georgakilas, AG; Sideris, EG

dc.contributor.author	Konsta, AA	en
dc.contributor.author	Visvardis, EE	en
dc.contributor.author	Haveles, KS	en
dc.contributor.author	Georgakilas, AG	en
dc.contributor.author	Sideris, EG	en
dc.date.accessioned	2014-03-01T02:42:05Z
dc.date.available	2014-03-01T02:42:05Z
dc.date.issued	2002	en
dc.identifier.issn	0022-3093	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/30776
dc.subject	Cell Membrane	en
dc.subject	Dielectric Properties	en
dc.subject	Dielectric Relaxation	en
dc.subject	Dna Damage	en
dc.subject	Dna Repair	en
dc.subject	Peripheral Blood Mononuclear Cell	en
dc.subject	Programmed Cell Death	en
dc.subject	Scanning Electron Microscopy	en
dc.subject.classification	Materials Science, Ceramics	en
dc.subject.classification	Materials Science, Multidisciplinary	en
dc.subject.other	Cell membranes	en
dc.subject.other	Dielectric properties	en
dc.subject.other	Electrophoresis	en
dc.subject.other	Fractionation	en
dc.subject.other	Scanning electron microscopy	en
dc.subject.other	Dielectric relaxation spectroscopy	en
dc.subject.other	DNA	en
dc.title	Detecting radiation-induced DNA damage: From changes in dielectric properties to programmed cell death	en
heal.type	conferenceItem	en
heal.identifier.primary	10.1016/S0022-3093(02)01102-X	en
heal.identifier.secondary	http://dx.doi.org/10.1016/S0022-3093(02)01102-X	en
heal.language	English	en
heal.publicationDate	2002	en
heal.abstract	The purpose of this work is to detect sequential processes leading, from the very early events induced from the deposition of energy on the DNA helix structure and associated with the release of Na+ counterions from the DNA macromolecule, to the disruption of the cell membrane, the final stage of the programmed cell death. To follow up the evolution of these sequential events, broadband dielectric relaxation spectroscopy was integrated in a series of techniques comprising, additionally, thermal transition spectroscopy, pulsed field gel elecrophoresis, multiple microgel 'comet assay', detection of caspases activation and DNA repair inhibition, and scanning electron microscopy. Using these techniques, the induction of DNA degradation and cell membrane disruption in human peripheral blood mononuclear cells was investigated. (C) 2002 Published by Elsevier Science B.V.	en
heal.publisher	ELSEVIER SCIENCE BV	en
heal.journalName	Journal of Non-Crystalline Solids	en
dc.identifier.doi	10.1016/S0022-3093(02)01102-X	en
dc.identifier.isi	ISI:000176261600038	en
dc.identifier.volume	305	en
dc.identifier.issue	1-3	en
dc.identifier.spage	295	en
dc.identifier.epage	302	en