Samarium-153 and technetium-99m-labeled monoclonal antibodies in angiogenesis for tumor visualization and inhibition

Bouziotis, P; Fani, M; Archimandritis, SC; Loundos, G; Paravatou, M; Bicknell, R; Harris, AL; Xanthopoulos, S; Stratis, N; Varvarigou, AD

dc.contributor.author	Bouziotis, P	en
dc.contributor.author	Fani, M	en
dc.contributor.author	Archimandritis, SC	en
dc.contributor.author	Loundos, G	en
dc.contributor.author	Paravatou, M	en
dc.contributor.author	Bicknell, R	en
dc.contributor.author	Harris, AL	en
dc.contributor.author	Xanthopoulos, S	en
dc.contributor.author	Stratis, N	en
dc.contributor.author	Varvarigou, AD	en
dc.date.accessioned	2014-03-01T02:42:20Z
dc.date.available	2014-03-01T02:42:20Z
dc.date.issued	2003	en
dc.identifier.issn	0250-7005	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/30951
dc.relation.uri	http://www.scopus.com/inward/record.url?eid=2-s2.0-0042306233&partnerID=40&md5=432922a23e8106f72b0b8ee903ed5d16	en
dc.subject	Angiogenesis	en
dc.subject	Monoclonal antibodies	en
dc.subject	Radioimmunoscintigraphy	en
dc.subject	Radioimmunotherapy	en
dc.subject	Samarium-153	en
dc.subject	Technetium-99m	en
dc.subject	VEGF	en
dc.subject.classification	Oncology	en
dc.subject.other	angiogenic factor	en
dc.subject.other	monoclonal antibody	en
dc.subject.other	monoclonal antibody VG76e	en
dc.subject.other	radioisotope	en
dc.subject.other	samarium 153	en
dc.subject.other	technetium 99m	en
dc.subject.other	unclassified drug	en
dc.subject.other	vasculotropin	en
dc.subject.other	vasculotropin receptor	en
dc.subject.other	angiogenesis	en
dc.subject.other	animal experiment	en
dc.subject.other	animal model	en
dc.subject.other	animal tissue	en
dc.subject.other	antibody labeling	en
dc.subject.other	cancer inhibition	en
dc.subject.other	conference paper	en
dc.subject.other	controlled study	en
dc.subject.other	diagnostic imaging	en
dc.subject.other	drug distribution	en
dc.subject.other	drug elimination	en
dc.subject.other	drug uptake	en
dc.subject.other	early diagnosis	en
dc.subject.other	evaluation	en
dc.subject.other	female	en
dc.subject.other	hepatobiliary system	en
dc.subject.other	human	en
dc.subject.other	human cell	en
dc.subject.other	isotope labeling	en
dc.subject.other	mouse	en
dc.subject.other	mouse strain	en
dc.subject.other	nonhuman	en
dc.subject.other	priority journal	en
dc.subject.other	protein analysis	en
dc.subject.other	protein function	en
dc.subject.other	tissue distribution	en
dc.subject.other	tumor diagnosis	en
dc.subject.other	tumor localization	en
dc.subject.other	tumor vascularization	en
dc.subject.other	Animals	en
dc.subject.other	Antibodies, Monoclonal	en
dc.subject.other	Breast Neoplasms	en
dc.subject.other	Female	en
dc.subject.other	Humans	en
dc.subject.other	Immunoconjugates	en
dc.subject.other	Isotope Labeling	en
dc.subject.other	Mice	en
dc.subject.other	Mice, Nude	en
dc.subject.other	Neovascularization, Pathologic	en
dc.subject.other	Radioisotopes	en
dc.subject.other	Radiopharmaceuticals	en
dc.subject.other	Samarium	en
dc.subject.other	Technetium	en
dc.subject.other	Tumor Cells, Cultured	en
dc.title	Samarium-153 and technetium-99m-labeled monoclonal antibodies in angiogenesis for tumor visualization and inhibition	en
heal.type	conferenceItem	en
heal.language	English	en
heal.publicationDate	2003	en
heal.abstract	Background: Angiogenesis is the development of new blood vessels from pre-existing ones. Vascular endothelial growth factor (VEGF) is one of the most important angiogenic activators. Our studies are focused on the detection of VEGF by use of radiolabeled anti-endothelial monoclonal antibodies, which have the ability to localize in newly-formed vasculature of a cancerous origin. Materials and Methods: The anti-endothelial monoclonal antibody VG76e was labeled with Samarium-153 and Technetium-99m. Biodistribution of the radiolabeled species was assessed in normal female Swiss mice, while tumor uptake was also evaluated. Results: VG76e was labeled with 99mTc and (153)sm, resulting in a single product with a labeling yield of over 95%. Biodistribution studies showed non-specific uptake in any organ, with elimination via the hepatobiliary system. Finally, satisfactory tumor uptake was observed for both radiolabeled derivatives. Conclusion: Monoclonal antibodies raised against epithelial growth factors or their receptors, when labeled with appropriate radionuclides, may be a useful tool for early tumor detection and eventually for therapy.	en
heal.publisher	INT INST ANTICANCER RESEARCH	en
heal.journalName	Anticancer Research	en
dc.identifier.isi	ISI:000184359100023	en
dc.identifier.volume	23	en
dc.identifier.issue	3 A	en
dc.identifier.spage	2167	en
dc.identifier.epage	2171	en