Dielectric study of the programmed cell death, apoptosis, in human leukemia

Kanapitsas, A; Vartzeli-Nikaki, P; Konsta, AA; Visvardis, E-E; Sideris, EG

dc.contributor.author	Kanapitsas, A	en
dc.contributor.author	Vartzeli-Nikaki, P	en
dc.contributor.author	Konsta, AA	en
dc.contributor.author	Visvardis, E-E	en
dc.contributor.author	Sideris, EG	en
dc.date.accessioned	2014-03-01T02:43:13Z
dc.date.available	2014-03-01T02:43:13Z
dc.date.issued	2005	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/31303
dc.subject	Biological Effect	en
dc.subject	Cell Membrane	en
dc.subject	Chronic Lymphocytic Leukemia	en
dc.subject	Dielectric Permittivity	en
dc.subject	Dielectric Properties	en
dc.subject	Dielectric Spectroscopy	en
dc.subject	Dna Damage	en
dc.subject	Programmed Cell Death	en
dc.subject	Alternating Field	en
dc.subject	Healthy Donors	en
dc.subject	Very Low Frequency	en
dc.subject.other	Cell death	en
dc.subject.other	Diseases	en
dc.subject.other	DNA	en
dc.subject.other	Electric conductivity	en
dc.subject.other	Spectroscopic analysis	en
dc.subject.other	Broadband Dielectric Spectroscopy (DRS)	en
dc.subject.other	Human leukemia	en
dc.subject.other	Lymphocytic leukemia	en
dc.subject.other	Weak stacking forces	en
dc.subject.other	Dielectric properties	en
dc.title	Dielectric study of the programmed cell death, apoptosis, in human leukemia	en
heal.type	conferenceItem	en
heal.identifier.primary	10.1109/ISE.2005.1612434	en
heal.identifier.secondary	http://dx.doi.org/10.1109/ISE.2005.1612434	en
heal.identifier.secondary	1612434	en
heal.publicationDate	2005	en
heal.abstract	Previous work in our laboratories showed that Broadband Dielectric Spectroscopy (DRS) reveals the very early physical events on damaged DNA which lead to the disruption of the DNA weak stacking forces. We followed up the evolution of this early physical damage into chemical damage within human cells. The induced chemical lesions result in biological effects including the initiation of programmed cell death, apoptosis, a mechanism resulting in extensive disruption of the external cell membranes. Using sensitive bio-methodologies we revealed that the early DNA damage was finally initiating apoptosis only when the damage was overwhelming intrinsic repair mechanisms. Finally, we were able to study the early stages of membrane alteration, associated with apoptosis, by measuring modifications in the dielectric properties of the cell membrane. It was found that in cells from donors with the chronic lymphocytic leukemia (CLL-cells), which exhibit a higher frequency of apoptosis, the values of the real and the imaginary part of the dielectric permittivity, e*, i.e. e′ and e″ respectively, and of the alternate field specific conductivity, sac, as a function of frequency, were higher than those of the healthy donors, in which apoptosis occurs at very low frequency.	en
heal.journalName	Proceedings - International Symposium on Electrets	en
dc.identifier.doi	10.1109/ISE.2005.1612434	en
dc.identifier.volume	2005	en
dc.identifier.spage	497	en
dc.identifier.epage	500	en