Dielectric properties during apoptosis in peripheral blood cells from chronic lymphocytic leukaemia patients

Kanapitsas, A; Vartzeli-Nikaki, P; Konsta, AA; Visvardis, E-E; Sideris, EG

dc.contributor.author	Kanapitsas, A	en
dc.contributor.author	Vartzeli-Nikaki, P	en
dc.contributor.author	Konsta, AA	en
dc.contributor.author	Visvardis, E-E	en
dc.contributor.author	Sideris, EG	en
dc.date.accessioned	2014-03-01T02:44:00Z
dc.date.available	2014-03-01T02:44:00Z
dc.date.issued	2006	en
dc.identifier.issn	1070-9878	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/31611
dc.subject	Apoptosis	en
dc.subject	Biological cells	en
dc.subject	Biomedical measurements	en
dc.subject	Biomembranes	en
dc.subject	Blood	en
dc.subject	Conductivity	en
dc.subject	Dielectric measurements	en
dc.subject	Leukaemia	en
dc.subject.classification	Engineering, Electrical & Electronic	en
dc.subject.classification	Physics, Applied	en
dc.subject.other	Blood	en
dc.subject.other	Cell membranes	en
dc.subject.other	Diseases	en
dc.subject.other	DNA	en
dc.subject.other	Patient treatment	en
dc.subject.other	Permittivity	en
dc.subject.other	Apoptosis	en
dc.subject.other	Biological cells	en
dc.subject.other	Dielectric measurements	en
dc.subject.other	Leukaemia	en
dc.subject.other	Cells	en
dc.title	Dielectric properties during apoptosis in peripheral blood cells from chronic lymphocytic leukaemia patients	en
heal.type	conferenceItem	en
heal.identifier.primary	10.1109/TDEI.2006.247832	en
heal.identifier.secondary	http://dx.doi.org/10.1109/TDEI.2006.247832	en
heal.identifier.secondary	1714930	en
heal.language	English	en
heal.publicationDate	2006	en
heal.abstract	Previous work in our laboratories showed that dielectric measurements reveal the very early physical events on damaged DNA which lead to the disruption of the DNA weak stacking forces. We followed up the evolution of this early physical damage into chemical damage within human cells, and were able to study the early stages of membrane alteration, associated with apoptosis, by measuring modifications in the dielectric properties of the cell membrane. It was found that in cells from patients with the B-chronic lymphocytic leukaemia (CLL-cells), which exhibit a higher rate of apoptosis, the values of the real and the imaginary part of the dielectric permittivity, epsilon*. i.e. epsilon' and epsilon" respectively, and of the alternate field specific conductivity, a,,, as a function of frequency, were higher than those of the healthy donors, in which apoptosis occurs at lower rate. Finally, large differences were found between the respective epsilon', epsilon" and sigma(ac), values observed in the cells isolated from the two leukaemic patients. These differences were matching the large differences observed between the corresponding apoptosis rates at the two leukaemic patients.	en
heal.publisher	IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC	en
heal.journalName	IEEE Transactions on Dielectrics and Electrical Insulation	en
dc.identifier.doi	10.1109/TDEI.2006.247832	en
dc.identifier.isi	ISI:000241254000016	en
dc.identifier.volume	13	en
dc.identifier.issue	5	en
dc.identifier.spage	1057	en
dc.identifier.epage	1062	en