Direct laser printing of biotin microarrays on low temperature oxide on Si substrates

Boutopoulos, C; Andreakou, P; Kafetzopoulos, D; Chatzandroulis, S; Zergioti, I

dc.contributor.author	Boutopoulos, C	en
dc.contributor.author	Andreakou, P	en
dc.contributor.author	Kafetzopoulos, D	en
dc.contributor.author	Chatzandroulis, S	en
dc.contributor.author	Zergioti, I	en
dc.date.accessioned	2014-03-01T02:45:14Z
dc.date.available	2014-03-01T02:45:14Z
dc.date.issued	2008	en
dc.identifier.issn	1862-6300	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/32222
dc.subject	Low Temperature	en
dc.subject.classification	Materials Science, Multidisciplinary	en
dc.subject.classification	Physics, Applied	en
dc.subject.classification	Physics, Condensed Matter	en
dc.subject.other	Beam sizes	en
dc.subject.other	Capacitive sensors	en
dc.subject.other	Direct laser printings	en
dc.subject.other	Direct printings	en
dc.subject.other	Direct-write	en
dc.subject.other	Electrical outputs	en
dc.subject.other	Energy densities	en
dc.subject.other	Energy density thresholds	en
dc.subject.other	Forward transfers	en
dc.subject.other	Laser beam parameters	en
dc.subject.other	Laser induced	en
dc.subject.other	Low temperatures	en
dc.subject.other	ND:YAG lasers	en
dc.subject.other	Passivation layers	en
dc.subject.other	Pulse durations	en
dc.subject.other	Si substrates	en
dc.subject.other	Spatial resolution.	en
dc.subject.other	Standard materials	en
dc.subject.other	Streptavidin	en
dc.subject.other	Surface functionalization	en
dc.subject.other	Biosensors	en
dc.subject.other	Coenzymes	en
dc.subject.other	Drop formation	en
dc.subject.other	Drops	en
dc.subject.other	Fluorescence microscopy	en
dc.subject.other	Lasers	en
dc.subject.other	Mass transfer	en
dc.subject.other	Microelectronics	en
dc.subject.other	Microscopic examination	en
dc.subject.other	Neodymium lasers	en
dc.subject.other	Passivation	en
dc.subject.other	Printing	en
dc.subject.other	Pulsed laser applications	en
dc.subject.other	Sensors	en
dc.subject.other	Silanes	en
dc.subject.other	Silicon	en
dc.subject.other	Substrates	en
dc.title	Direct laser printing of biotin microarrays on low temperature oxide on Si substrates	en
heal.type	conferenceItem	en
heal.identifier.primary	10.1002/pssa.200780206	en
heal.identifier.secondary	http://dx.doi.org/10.1002/pssa.200780206	en
heal.language	English	en
heal.publicationDate	2008	en
heal.abstract	Laser Induced Forward Transfer (LIFT) technique is a direct-write method allowing the effective deposition of a wide range of materials with high spatial resolution. In this work, we present the direct printing of microarrays from a biotin solution by using a Nd:YAG laser (266nm wavelength, 4ns pulse duration). Standard materials of microelectronics such as Si and Low Temperature Oxide on Si (LTO/Si) were used as receiving substrates. For the LTO substrate, a detailed study for the optimum surface functionalization was carried out as it is a common passivation layer for biosensors. The laser beam parameters, such as the energy density and the beam size, were systematically varied in order to evaluate the effect on the transferred droplets. Furthermore, the energy density threshold for the ejection of the droplets was defined. Finally, the viability of the deposited droplets was demonstrated through the biotin-streptavidin reaction on silane coated LTO/Si substrates. This reaction was confirmed by fluorescence microscopy. These results have shown that the LIFT process can be used for biosensors and possibly for label-free detection by the combination of the method with capacitive sensors, sensors based on cantilevers or other sensors with electrical output. (C) 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim	en
heal.publisher	WILEY-V C H VERLAG GMBH	en
heal.journalName	Physica Status Solidi (A) Applications and Materials Science	en
dc.identifier.doi	10.1002/pssa.200780206	en
dc.identifier.isi	ISI:000261088200002	en
dc.identifier.volume	205	en
dc.identifier.issue	11	en
dc.identifier.spage	2505	en
dc.identifier.epage	2508	en