Mechanical stability determines stress fiber and focal adhesion orientation

Stamenovic, D; Lazopoulos, KA; Pirentis, A; Suki, B

dc.contributor.author	Stamenovic, D	en
dc.contributor.author	Lazopoulos, KA	en
dc.contributor.author	Pirentis, A	en
dc.contributor.author	Suki, B	en
dc.date.accessioned	2014-03-01T02:46:13Z
dc.date.available	2014-03-01T02:46:13Z
dc.date.issued	2009	en
dc.identifier.issn	1865-5025	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/32611
dc.subject	Cytoskeleton	en
dc.subject	Elasticity	en
dc.subject	Focal adhesions	en
dc.subject	Potential	en
dc.subject	Stability	en
dc.subject	Stress fiber	en
dc.subject	Substrate stretching	en
dc.subject.other	conference paper	en
dc.subject.other	cytoskeleton	en
dc.subject.other	elasticity	en
dc.subject.other	energy	en
dc.subject.other	experimental study	en
dc.subject.other	feasibility study	en
dc.subject.other	focal adhesion	en
dc.subject.other	geometry	en
dc.subject.other	mathematical model	en
dc.subject.other	mechanical stability	en
dc.subject.other	medical literature	en
dc.subject.other	model	en
dc.subject.other	physical parameters	en
dc.subject.other	prediction	en
dc.subject.other	priority journal	en
dc.subject.other	stress fiber	en
dc.subject.other	stretching	en
dc.title	Mechanical stability determines stress fiber and focal adhesion orientation	en
heal.type	conferenceItem	en
heal.identifier.primary	10.1007/s12195-009-0093-3	en
heal.identifier.secondary	http://dx.doi.org/10.1007/s12195-009-0093-3	en
heal.language	English	en
heal.publicationDate	2009	en
heal.abstract	It is well documented in a variety of adherent cell types that in response to anisotropic signals from the microenvironment cells alter their cytoskeletal organization. Previous theoretical studies of these phenomena were focused primarily on the elasticity of cytoskeletal actin stress fibers (SFs) and of the substrate while the contribution of focal adhesions (FAs) through which the cytoskeleton is linked to the external environment has not been considered. Here we propose a mathematical model comprised of a single linearly elastic SF and two identical linearly elastic FAs of a finite size at the endpoints of the SF to investigate cytoskeletal realignment in response to uniaxial stretching of the substrate. The model also includes the contribution of the chemical potential energies of the SF and the FAs to the total potential energy of the SF-FA assembly. Using the global (Maxwell's) stability criterion, we predict stable configurations of the SF-FA assembly in response to substrate stretching. Model predictions obtained for physiologically feasible values of model parameters are consistent with experimental data from the literature. The model shows that elasticity of SFs alone can not predict their realignment during substrate stretching and that geometrical and elastic properties of SFs and FAs need to be included. © 2009 Biomedical Engineering Society.	en
heal.publisher	SPRINGER	en
heal.journalName	Cellular and Molecular Bioengineering	en
dc.identifier.doi	10.1007/s12195-009-0093-3	en
dc.identifier.isi	ISI:000272671600003	en
dc.identifier.volume	2	en
dc.identifier.issue	4	en
dc.identifier.spage	475	en
dc.identifier.epage	485	en