Combined information from AFM imaging and SHG signal analysis of collagen thin films

Stylianou, A; Politopoulos, K; Kyriazi, M; Yova, D

dc.contributor.author	Stylianou, A	en
dc.contributor.author	Politopoulos, K	en
dc.contributor.author	Kyriazi, M	en
dc.contributor.author	Yova, D	en
dc.date.accessioned	2014-03-01T02:47:19Z
dc.date.available	2014-03-01T02:47:19Z
dc.date.issued	2011	en
dc.identifier.issn	1746-8094	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/33067
dc.subject	Atomic force microscopy (AFM)	en
dc.subject	Collagen	en
dc.subject	Nonlinear optics (NLO)	en
dc.subject	Second harmonic generation (SHG)	en
dc.subject	Thin films	en
dc.subject.other	AFM imaging	en
dc.subject.other	Coating procedures	en
dc.subject.other	Collagen fiber structure	en
dc.subject.other	Collagen films	en
dc.subject.other	Collagen modification	en
dc.subject.other	Collagen structure	en
dc.subject.other	Combined informations	en
dc.subject.other	Hydrodynamic flows	en
dc.subject.other	Nanoscale resolutions	en
dc.subject.other	NLO properties	en
dc.subject.other	Non-invasive	en
dc.subject.other	Nonlinear optics (NLO)	en
dc.subject.other	Optical second-harmonic generation	en
dc.subject.other	Pathological conditions	en
dc.subject.other	Second harmonic generation (SHG)	en
dc.subject.other	Second harmonics	en
dc.subject.other	SHG signals	en
dc.subject.other	Thermal denaturations	en
dc.subject.other	Atomic force microscopy	en
dc.subject.other	Harmonic analysis	en
dc.subject.other	Harmonic generation	en
dc.subject.other	Mammals	en
dc.subject.other	Nonlinear optics	en
dc.subject.other	Optical properties	en
dc.subject.other	Pyrolysis	en
dc.subject.other	Spinning (fibers)	en
dc.subject.other	Thin films	en
dc.subject.other	Collagen	en
dc.subject.other	collagen	en
dc.subject.other	collagen fiber	en
dc.subject.other	analytic method	en
dc.subject.other	atomic force microscopy	en
dc.subject.other	conference paper	en
dc.subject.other	denaturation	en
dc.subject.other	film	en
dc.subject.other	film coating	en
dc.subject.other	hydrodynamics	en
dc.subject.other	nonlinear optics	en
dc.subject.other	optics	en
dc.subject.other	priority journal	en
dc.subject.other	second harmonic generation	en
dc.subject.other	signal transduction	en
dc.subject.other	thermal analysis	en
dc.subject.other	Mammalia	en
dc.title	Combined information from AFM imaging and SHG signal analysis of collagen thin films	en
heal.type	conferenceItem	en
heal.identifier.primary	10.1016/j.bspc.2011.02.006	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.bspc.2011.02.006	en
heal.language	English	en
heal.publicationDate	2011	en
heal.abstract	Collagen being the most abundant protein in mammals is important for a variety of functions and its structure, concentration and orientation disturbance is associated with different pathological states. The use of the optical second harmonic generation (SHG) is emerging as a powerful non-invasive tool for assessing collagen modification in a variety of pathological conditions. The properties of second harmonic light from collagen structures have not yet been fully clarified due to a number of limitations, such as the difficulty to prepare collagen samples with well-known characteristics and optical properties, at a nanoscale resolution. The results of this paper suggest that some of these limitations can be overcome by using thin collagen films with pre-determined characteristics (PDC), which maintain or/and enhance their NLO properties capacities, as they have been checked by using atomic force microscopy (AFM). The collagen fiber structure and orientation was systematically altered by using thermal denaturation or different preparation methodologies (spin coating procedure, use of collagen solution hydrodynamic flow). These films can be used as prototypes and the combined information from AFM imaging and the one included in SHG signal, delivered from them, can significantly contribute to further understanding of the NLO properties of collagen and in the long term to take advantage as a non-invasive tool. (C) 2011 Elsevier Ltd. All rights reserved.	en
heal.publisher	ELSEVIER SCI LTD	en
heal.journalName	Biomedical Signal Processing and Control	en
dc.identifier.doi	10.1016/j.bspc.2011.02.006	en
dc.identifier.isi	ISI:000293480100012	en
dc.identifier.volume	6	en
dc.identifier.issue	3	en
dc.identifier.spage	307	en
dc.identifier.epage	313	en