In-vitro effects of UVA-laser radiation on cells

Yova, Dido; Halkiotis, Konstantinos; Pantelias, Gabriel

dc.contributor.author	Yova, Dido	en
dc.contributor.author	Halkiotis, Konstantinos	en
dc.contributor.author	Pantelias, Gabriel	en
dc.date.accessioned	2014-03-01T02:48:24Z
dc.date.available	2014-03-01T02:48:24Z
dc.date.issued	1996	en
dc.identifier.issn	0277786X	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/33801
dc.relation.uri	http://www.scopus.com/inward/record.url?eid=2-s2.0-0029774691&partnerID=40&md5=485b588bfeba80bb5fe30857bf59eefc	en
dc.subject.other	Chinese hamster	en
dc.subject.other	In vivo measurements	en
dc.subject.other	Trypan blue test	en
dc.subject.other	Cells	en
dc.subject.other	DNA	en
dc.subject.other	Magnetic field effects	en
dc.subject.other	Q switched lasers	en
dc.subject.other	Ultraviolet radiation	en
dc.subject.other	Laser tissue interaction	en
dc.title	In-vitro effects of UVA-laser radiation on cells	en
heal.type	conferenceItem	en
heal.publicationDate	1996	en
heal.abstract	The widespread use of lasers in medicine has raised the awareness about the mutagenic effect of ultraviolet laser radiation. Although it is well known that the major target for UVC (200 - 290 nm) and UVB (290 - 320 nm) wavebands on cells and tissues is nuclear DNA, the information about UVA radiation (320 - 400 nm) is controversial, as far as some researchers present evidence that this waveband should be 1000-fold less cytotoxic and mutagenic than UVC, while others present evidence about its lethality and mutagenicity. In addition the mechanism of cell killing in the UVA range remains unclear. This work presents an extensive study of UVA-laser radiation (355 nm) effects on Chinese hamster ovary cells. The experiments were performed with an Nd:YAG/KD*P laser (third harmonic, 355 nm), operating at the Q-switch mode, 6 nsec pulse duration. Chinese hamster ovary cells (CHO cells) from a frozen cell line, were suspended in McCoy's 5A culture medium, washed with PBS, and after centrifuging at 200 g the pellet irradiated by UVA-laser beam. Cell viability studies were done in a range of fluences from 0 - 75 J/cm2, 5 Hz repetition rate. Direct effects of laser irradiation were evaluated by the Trypan Blue test, and post-effects by the cell survival assay. The degree of cell damage was related to the fluence and it was concluded that increasing fluences of radiation resulted in decrease rate of cell survival. Also, it was noticed that the survival fraction 8 days after irradiation was decreased in relation to the survival fraction just after irradiation. Direct and post-effects are discussed and related with pertinent mechanisms of damage and repair by UVA light.	en
heal.journalName	Proceedings of SPIE - The International Society for Optical Engineering	en
dc.identifier.volume	2630	en
dc.identifier.spage	125	en
dc.identifier.epage	133	en