Intracellular photoinduced oxidative stress by zinc phthalocyanine photosensitization. A study of the early events in real time using confocal microscopy

Alexandratou, E; Yova, D; Handris, P; Kletsas, D; Loukas, S

dc.contributor.author	Alexandratou, E	en
dc.contributor.author	Yova, D	en
dc.contributor.author	Handris, P	en
dc.contributor.author	Kletsas, D	en
dc.contributor.author	Loukas, S	en
dc.date.accessioned	2014-03-01T02:49:25Z
dc.date.available	2014-03-01T02:49:25Z
dc.date.issued	2003	en
dc.identifier.issn	0277786X	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/34590
dc.relation.uri	http://www.scopus.com/inward/record.url?eid=2-s2.0-1342309849&partnerID=40&md5=8cdf7a628004dcd475d9bd9073d635d4	en
dc.subject	Confocal microscopy	en
dc.subject	Oxidative stress	en
dc.subject	Phthalocyanine	en
dc.subject	Real time	en
dc.subject	Single cell level	en
dc.subject.other	Cell membranes	en
dc.subject.other	Cells	en
dc.subject.other	Cytology	en
dc.subject.other	Fluorescence	en
dc.subject.other	Microscopic examination	en
dc.subject.other	Pathology	en
dc.subject.other	Photosensitivity	en
dc.subject.other	Probes	en
dc.subject.other	Real time systems	en
dc.subject.other	Confocal microscopy	en
dc.subject.other	Human fibroblasts	en
dc.subject.other	Intracellular pH	en
dc.subject.other	Mitochondira membrane	en
dc.subject.other	Oxidative stress	en
dc.subject.other	Photodynamic doze	en
dc.subject.other	Reactive oxygen species	en
dc.subject.other	Zinc phthalocyanine photosensitization	en
dc.subject.other	Photodynamic therapy	en
dc.title	Intracellular photoinduced oxidative stress by zinc phthalocyanine photosensitization. A study of the early events in real time using confocal microscopy	en
heal.type	conferenceItem	en
heal.publicationDate	2003	en
heal.abstract	Oxidative stress has been implicated in several biological and pathological aspects. Reactive oxygen species (ROS) have been proposed to act as signal transduction molecules activating reactions leading to cell rescue or to cell apoptosis/necrosis. In the present study, oxidative stress was induced by photosensitization of zinc phthalocyanine (ZnPc) in human fibroblasts using a photodynamic dose that did not lead to apoptosis or necrosis. The induction of oxidative stress was performed at the microscope stage in preassigned time. The cascade of phenomena evoked was studied in real time and at the single cell level using confocal laser scanning microscopy. Using specific vital fluorescent probes, alterations induced by oxidative stress in mitochondria membrane potential, in intracellular pH and in calcium concentration were recorded. Image processing and analysis techniques were used to quantify the observed changes. Subcellular localization of the photosensitizer was studied in order to determine the primary and immediate ROS target. It was found that ZnPc is mainly localized in the mitochondria region.	en
heal.journalName	Proceedings of SPIE - The International Society for Optical Engineering	en
dc.identifier.volume	5142	en
dc.identifier.spage	1	en
dc.identifier.epage	8	en