Advances in laser and tissue interactions: Laser microbeams and optical trapping

Serafetinides, AA; Makropoulou, M; Papadopoulos, D; Papagiakoumou, E; Pietreanu, D

dc.contributor.author	Serafetinides, AA	en
dc.contributor.author	Makropoulou, M	en
dc.contributor.author	Papadopoulos, D	en
dc.contributor.author	Papagiakoumou, E	en
dc.contributor.author	Pietreanu, D	en
dc.date.accessioned	2014-03-01T02:49:59Z
dc.date.available	2014-03-01T02:49:59Z
dc.date.issued	2005	en
dc.identifier.issn	0277786X	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/34849
dc.subject.other	Laser ablation	en
dc.subject.other	Laser beams	en
dc.subject.other	Tissue	en
dc.subject.other	Biological tissues	en
dc.subject.other	Diagnostic techniques	en
dc.subject.other	Optical trapping devices	en
dc.subject.other	Lasers	en
dc.title	Advances in laser and tissue interactions: Laser microbeams and optical trapping	en
heal.type	conferenceItem	en
heal.identifier.primary	10.1117/12.618427	en
heal.identifier.secondary	http://dx.doi.org/10.1117/12.618427	en
heal.identifier.secondary	61	en
heal.publicationDate	2005	en
heal.abstract	The increasing use of lasers in biomedical research and clinical praxis leads to the development and application of new, non-invasive, therapeutic, surgical and diagnostic techniques. In laser surgery, the theory of ablation dictates that pulsed mid-infrared laser beams exhibit strong absorption by soft and hard tissues, restricting residual thermal damage to a minimum zone. Therefore, the development of high quality 3 μm lasers is considered to be an alternative for precise laser ablation of tissue. Among them are the high quality oscillator-two stages amplifier lasers developed, which will be described in this article. The beam quality delivered by these lasers to the biological tissue is of great importance in cutting and ablating operations. As the precision of the ablation is increased, the cutting laser interventions could well move to the microsurgery field. Recently, the combination of a laser scalpel with an optical trapping device, under microscopy control, is becoming increasingly important Optical manipulation of microscopic particles by focused laser beams, is now widely used as a powerful tool for 'non-contact' micromanipulation of cells and organelles. Several laser sources are employed for trapping and varying laser powers are used in a broad range of applications of optical tweezers. For most of the lasers used, the focal spot of the trapping beam is of the order of a micron. As the trapped objects can vary in size from hundreds of nanometres to hundreds of microns, the technique has recently invaded into the nanocosmos of genes and molecules. However, the use of optical trapping for quantitative research into biophysical processes requires accurate calculation of the optical forces and torques acting within the trap. The research and development efforts towards a mid-IR microbeam laser system, the design and realization efforts towards a visible laser trapping system and the first results obtained using a relatively new calibration method to calculate the forces experienced in the optical trap are discussed in detail in the following.	en
heal.journalName	Proceedings of SPIE - The International Society for Optical Engineering	en
dc.identifier.doi	10.1117/12.618427	en
dc.identifier.volume	5830	en
dc.identifier.spage	357	en
dc.identifier.epage	367	en