Cell sorting in a static optical potential landscape

Paterson, L; Papagiakoumou, E; Milne, G; Garces-Chavez, V; Riches, AC; Dholakia, K

dc.contributor.author	Paterson, L	en
dc.contributor.author	Papagiakoumou, E	en
dc.contributor.author	Milne, G	en
dc.contributor.author	Garces-Chavez, V	en
dc.contributor.author	Riches, AC	en
dc.contributor.author	Dholakia, K	en
dc.date.accessioned	2014-03-01T02:50:00Z
dc.date.available	2014-03-01T02:50:00Z
dc.date.issued	2005	en
dc.identifier.issn	0277786X	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/34867
dc.subject	Bessel beam	en
dc.subject	Cell sorting	en
dc.subject	Erythrocytes	en
dc.subject	Lymphocytes	en
dc.subject	Optical manipulation	en
dc.subject.other	Biotechnology	en
dc.subject.other	Blood	en
dc.subject.other	Brownian movement	en
dc.subject.other	Diffraction	en
dc.subject.other	Optical properties	en
dc.subject.other	Bessel beams	en
dc.subject.other	Cell sorting	en
dc.subject.other	Erythrocytes	en
dc.subject.other	Lymphocytes	en
dc.subject.other	Optical manipulation	en
dc.subject.other	Cells	en
dc.title	Cell sorting in a static optical potential landscape	en
heal.type	conferenceItem	en
heal.identifier.primary	10.1117/12.614971	en
heal.identifier.secondary	http://dx.doi.org/10.1117/12.614971	en
heal.identifier.secondary	59301M	en
heal.publicationDate	2005	en
heal.abstract	Microscopic particles with varying optical properties may be induced to move in different ways when placed on a sculpted optical potential due to differences in shape, size or polarisability. The separation of red blood cells (erythrocytes) and white blood cells (lymphocytes) is achieved in a non-invasive manner and in the absence of any microfluidic systems using a 'non-diffracting' circularly symmetric Bessel beam. The Bessel beam, which consists of a series of concentric rings, each of equal power and of 3.2μm thickness with a spacing of 2μm around a central maximum of 5μm diameter (and is akin to a rod of light as its propagation distance is 3mm), is directed upward into a sample chamber containing blood. Fluctuations in Brownian motion cause cells to escape from individual rings of the Bessel beam and travel towards the beam centre, where the intensity of the rings increases. However, these cells must be able to overcome the potential barrier of each ring which gets larger toward the central maximum. Lymphocytes - spherical in shape and 7μm in diameter (therefore overlapping two rings) - are transported, due to the gradient force of the optical field, to the beam centre where they are guided upwards and form a vertical stack, whereas erythrocytes re-align on their sides in the outer rings and are then guided upwards, because once aligned they cannot escape the potential barrier and 'lock-in' to that ring. The optical power required for optimal sorting in this static sorter which requires no fluid flow is investigated.	en
heal.journalName	Proceedings of SPIE - The International Society for Optical Engineering	en
dc.identifier.doi	10.1117/12.614971	en
dc.identifier.volume	5930	en
dc.identifier.spage	1	en
dc.identifier.epage	8	en