Distribution studies of m-THPC after topical application of m-THPC thermogel in a murine non-melanoma side cancer tumor model by fluorescence spectroscopic and imaging techniques

Alexandratou, E; Kyriazi, M; Yova, D; Grafe, S; Trebst, T; Johansson, A; Svensson, J; Svanberg, K; Bendsoe, N; Anderson-Engels, S

dc.contributor.author	Alexandratou, E	en
dc.contributor.author	Kyriazi, M	en
dc.contributor.author	Yova, D	en
dc.contributor.author	Grafe, S	en
dc.contributor.author	Trebst, T	en
dc.contributor.author	Johansson, A	en
dc.contributor.author	Svensson, J	en
dc.contributor.author	Svanberg, K	en
dc.contributor.author	Bendsoe, N	en
dc.contributor.author	Anderson-Engels, S	en
dc.date.accessioned	2014-03-01T02:50:20Z
dc.date.available	2014-03-01T02:50:20Z
dc.date.issued	2006	en
dc.identifier.issn	16057422	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/35073
dc.subject	Biodistribution	en
dc.subject	Fluorescence imaging	en
dc.subject	Fluorescence spectroscopy	en
dc.subject	m-THPC thermogel pharmacokinetics	en
dc.subject	Photodynamic therapy	en
dc.subject.other	Fluorescence	en
dc.subject.other	Imaging techniques	en
dc.subject.other	Mathematical models	en
dc.subject.other	Muscle	en
dc.subject.other	Photosensitizers	en
dc.subject.other	Skin	en
dc.subject.other	Spectroscopic analysis	en
dc.subject.other	Tumors	en
dc.subject.other	Biodistribution	en
dc.subject.other	Emission wavelengths	en
dc.subject.other	Skin carcinomas	en
dc.subject.other	Thermogels	en
dc.subject.other	Photodynamic therapy	en
dc.title	Distribution studies of m-THPC after topical application of m-THPC thermogel in a murine non-melanoma side cancer tumor model by fluorescence spectroscopic and imaging techniques	en
heal.type	conferenceItem	en
heal.identifier.primary	10.1117/12.646142	en
heal.identifier.secondary	http://dx.doi.org/10.1117/12.646142	en
heal.identifier.secondary	61390G	en
heal.publicationDate	2006	en
heal.abstract	m-THPC photodynamic therapy has been successfully studied in skin cancer, but no research effort concerning its topical application has been performed until now. Determination of the biodistribution of a special m-THPC thermogel formulation and its tumour selectivity was studied after topical application on hairless SKH-1 mice bearing nonmelanoma skin carcinomas. 20 ul/cm2 of m-THPC thermogel (0.5 mg m-THPC/ml) were applied on normal and tumour area and the concentration or demarcation of tumor by mTHPC fluorescence was measured at 2, 4 and 6 hours after drug application by three methods: 1. A fluorescence imaging system capturing images at two emission wavelengths (500 and 654 nm) following 405 nm excitation. Signals from different regions of interest were averaged and the intensity ratio at 654 to 500 was calculated. 2. A fluorescence spectrometer with a fiber bundle for in vivo spectra recording after 420 nm excitation. 3 Each animal was euthanized and the photosensitizer was chemically extracted from liver, spleen, muscle, normal skin and tumour. The photosensitizer concentrations in the extracts and in plasma were determined by fluorescence spectroscopy. The in vivo methods showed a remarkable difference in the concentration of photosensitizer in normal skin and tumour. The highest concentration in tumour was observed 6h after drug application and the highest fluorescence intensity ratio of m-THPC in tumour to normal tissue was observed at 4 hours. Furthermore, no m-THPC was detected in normal tissues or plasma after drug topical application. In vivo and ex vivo results were consistent.	en
heal.journalName	Progress in Biomedical Optics and Imaging - Proceedings of SPIE	en
dc.identifier.doi	10.1117/12.646142	en
dc.identifier.volume	6139	en