Optical trapping forces calculation by dielectrophoresis

Papagiakoumou, E; Makropoulou, MI; Perakis, D; Pietreanu, D; Kovacs, E; Serafetinides, AA

dc.contributor.author	Papagiakoumou, E	en
dc.contributor.author	Makropoulou, MI	en
dc.contributor.author	Perakis, D	en
dc.contributor.author	Pietreanu, D	en
dc.contributor.author	Kovacs, E	en
dc.contributor.author	Serafetinides, AA	en
dc.date.accessioned	2014-03-01T02:50:48Z
dc.date.available	2014-03-01T02:50:48Z
dc.date.issued	2006	en
dc.identifier.issn	0277786X	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/35156
dc.subject	Dielectrophoresis	en
dc.subject	Force measurements	en
dc.subject	Optical tweezers	en
dc.subject.other	Algorithms	en
dc.subject.other	Calibration	en
dc.subject.other	Electric field effects	en
dc.subject.other	Electrodes	en
dc.subject.other	Force measurement	en
dc.subject.other	Green's function	en
dc.subject.other	Lasers	en
dc.subject.other	Polystyrenes	en
dc.subject.other	Dielectrophoresis	en
dc.subject.other	Laser trapping system	en
dc.subject.other	Optical tweezers	en
dc.subject.other	Polystyrene spheres	en
dc.subject.other	Electrophoresis	en
dc.title	Optical trapping forces calculation by dielectrophoresis	en
heal.type	conferenceItem	en
heal.identifier.primary	10.1117/12.714153	en
heal.identifier.secondary	http://dx.doi.org/10.1117/12.714153	en
heal.identifier.secondary	62840D	en
heal.publicationDate	2006	en
heal.abstract	In this work we present a laser trapping system based on an Ar+ laser and the calibration method we used for the optical trapping force measurements on polystyrene spheres. The method uses the dielectrophoretic force exerted on a dielectric particle from an inhomogeneous electric field for the calculation of the optical force. We used polystyrene microspheres on a dielectrophoretic chamber with planar electrodes in the optical tweezers setup. For the determination of the dielectrophoretic force the method is based on Green's theorem. A sphere is trapped in the optical tweezers at various laser powers. An electrical field is applied between the electrodes and thus a dielectrophoretic force is exerted on the particle. The electric field is increased very slowly until the cell is pulled out from the trap. At this moment the optical force equals the dielectrophoretic force, which can be theoretically calculated. This algorithm is repeated several times and the optical force is graphically represented as a function of laser power. Forces of some pN were calculated.	en
heal.journalName	Proceedings of SPIE - The International Society for Optical Engineering	en
dc.identifier.doi	10.1117/12.714153	en
dc.identifier.volume	6284	en