Ultra-violet laser microbeam and optical trapping for cell micromanipulation

Kotsifaki, D; Makropoulou, M; Serafetinides, AA

dc.contributor.author	Kotsifaki, D	en
dc.contributor.author	Makropoulou, M	en
dc.contributor.author	Serafetinides, AA	en
dc.date.accessioned	2014-03-01T02:51:17Z
dc.date.available	2014-03-01T02:51:17Z
dc.date.issued	2007	en
dc.identifier.issn	16057422	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/35448
dc.subject	He-Ne laser	en
dc.subject	Laser ablation	en
dc.subject	N2 laser	en
dc.subject	Optical tweezers	en
dc.subject	Yeast cells	en
dc.subject.other	Cells	en
dc.subject.other	Helium neon lasers	en
dc.subject.other	Laser ablation	en
dc.subject.other	Optical tweezers	en
dc.subject.other	Yeast	en
dc.subject.other	Laser microbeams	en
dc.subject.other	Microdissection	en
dc.subject.other	Micromanipulation	en
dc.subject.other	Optical trapping	en
dc.subject.other	Laser beams	en
dc.title	Ultra-violet laser microbeam and optical trapping for cell micromanipulation	en
heal.type	conferenceItem	en
heal.identifier.primary	10.1117/12.741079	en
heal.identifier.secondary	http://dx.doi.org/10.1117/12.741079	en
heal.identifier.secondary	65351O	en
heal.publicationDate	2007	en
heal.abstract	In recent years, lasers for optical trapping and micromanipulation of microscopic particles or cells and subcellular structures, both in vivo and in vitro, have gained remarkable interest in biomedical research and applications. On the other hand, a highly focused pulsed laser allows ablation and microdissection of biological material with high spatial resolution. In 1989 the microbeam field and the optical trapping field were merged by the first combination of laser microbeams and optical tweezers. Several laser sources are employed for the combination of a laser scalpel with an optical trapping device, under microscopic control. For example a pulsed laser microbeam that emits in the ultraviolet or the visible range of the electromagnetic spectrum can be used to form submicrometer cuts in biological material or to ablate parts specific cells. However, although the principles and the mechanisms of pulsed laser ablation have been well described for macroscopic interventions, the microbeam operation under microscopic guidance necessitates further investigation. In this work, we present the research and development efforts towards a pulsed UV microbeam laser system, the design and realization efforts towards a visible laser trapping system and the first results obtained on yeast cells by the combined system. The nitrogen laser microbeam setup, with special UV mirrors, lenses and a microscope in which ablation and the He-Ne optical tweezers are combined, performs the microsurgery and micromanipulation. This UV laser based setup provides good spatial resolution for microdissection. The beam quality delivered by the laser is of great importance in microscopy controlled ablating operations and therefore was extensively studied.	en
heal.journalName	Progress in Biomedical Optics and Imaging - Proceedings of SPIE	en
dc.identifier.doi	10.1117/12.741079	en
dc.identifier.volume	6535	en