Evaluation of the PDT effect of Foscan® and Fospeg® in the LNCaP human prostate cancer cell line

Petri, A; Kyriazi, M; Alexandratou, E; Rallis, M; Grafe, S; Yova, D

dc.contributor.author	Petri, A	en
dc.contributor.author	Kyriazi, M	en
dc.contributor.author	Alexandratou, E	en
dc.contributor.author	Rallis, M	en
dc.contributor.author	Grafe, S	en
dc.contributor.author	Yova, D	en
dc.date.accessioned	2014-03-01T02:51:59Z
dc.date.available	2014-03-01T02:51:59Z
dc.date.issued	2009	en
dc.identifier.issn	16057422	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/35797
dc.subject	Confocal microscopy	en
dc.subject	Foscan®	en
dc.subject	Fospeg®	en
dc.subject	In vitro PDT	en
dc.subject.other	Cellular death	en
dc.subject.other	Cellular viability	en
dc.subject.other	Cytotoxic	en
dc.subject.other	Cytotoxic effects	en
dc.subject.other	Experimental conditions	en
dc.subject.other	Fluorescence intensities	en
dc.subject.other	Human prostate cancer cells	en
dc.subject.other	In vitro PDT	en
dc.subject.other	Intracellular localization	en
dc.subject.other	Laser scanning confocal microscopy	en
dc.subject.other	Liposomal formulation	en
dc.subject.other	Photodynamic treatments	en
dc.subject.other	Cell culture	en
dc.subject.other	Cell membranes	en
dc.subject.other	Confocal microscopy	en
dc.subject.other	Drug products	en
dc.subject.other	Irradiation	en
dc.subject.other	Laser tissue interaction	en
dc.subject.other	Photosensitizers	en
dc.subject.other	Lasers	en
dc.title	Evaluation of the PDT effect of Foscan® and Fospeg® in the LNCaP human prostate cancer cell line	en
heal.type	conferenceItem	en
heal.identifier.primary	10.1117/12.831881	en
heal.identifier.secondary	http://dx.doi.org/10.1117/12.831881	en
heal.identifier.secondary	73731I	en
heal.publicationDate	2009	en
heal.abstract	In this paper the cytotoxic effect of m-THPC, Foscan®, as well as of the liposomal formulation of m-THPC, Fospeg®, (kind offer of Biolitec) were studied post PDT in the human prostate cancer cell line LNCaP. The cells were incubated for 24h with 0.15 μg/ml and 1.2 μg/ml Foscan® and Fospeg®. Irradiation was performed with a 652nm laser and energy doses 180, 360 and 540mJ/cm2. The effect was assessed by the MTT viability test 24h after irradiation. Also the intracellular localization of Foscan® and Fospeg® was monitored by using Laser Scanning Confocal Microscopy Imaging. The results showed no dark toxicity either with Foscan® or Fospeg® at any concentration. Also irradiation at each energy dose in the absence of any photosensitizer, did not affect cellular viability. The cellular death caused after Photodynamic Treatment was dependent on m-THPC concentration and formulation, as well as the delivered energy dose. Fospeg® was more effective as LD50 was achieved with 0.15μg/ml at 180mJ/cm2 while for the same cytotoxic result 1.2μg/ml Foscan® was needed. Images from confocal microscopy revealed higher fluorescence intensity in the cytoplasm after incubation with Fospeg®, than upon incubation with Foscan® under the same experimental conditions. © 2009 SPIE-OSA.	en
heal.journalName	Progress in Biomedical Optics and Imaging - Proceedings of SPIE	en
dc.identifier.doi	10.1117/12.831881	en
dc.identifier.volume	7373	en