New approach in prostate Gleason grading using fluorescence microscopic imaging

Alexandratou, E; Yova, D; Gorpas, D

dc.contributor.author	Alexandratou, E	en
dc.contributor.author	Yova, D	en
dc.contributor.author	Gorpas, D	en
dc.date.accessioned	2014-03-01T02:52:04Z
dc.date.available	2014-03-01T02:52:04Z
dc.date.issued	2009	en
dc.identifier.issn	16057422	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/35826
dc.subject	Colocalisation metrics	en
dc.subject	Fluorescent probes	en
dc.subject	Gleason grading	en
dc.subject	Image analysis	en
dc.subject	Prostate cancer	en
dc.subject.other	Binding pattern	en
dc.subject.other	Colocalisation	en
dc.subject.other	Colocalisation metrics	en
dc.subject.other	Fluorescent probes	en
dc.subject.other	Gleason grading	en
dc.subject.other	Gold standards	en
dc.subject.other	Image processing and analysis	en
dc.subject.other	Intermediate stage	en
dc.subject.other	Localisation	en
dc.subject.other	Microscopic imaging	en
dc.subject.other	New approaches	en
dc.subject.other	Propidium iodide	en
dc.subject.other	Prostate cancer	en
dc.subject.other	Prostate cancers	en
dc.subject.other	Sialic acids	en
dc.subject.other	Biopolymers	en
dc.subject.other	Carboxylic acids	en
dc.subject.other	Confocal microscopy	en
dc.subject.other	Fluorescence	en
dc.subject.other	Fluorescence spectroscopy	en
dc.subject.other	Image analysis	en
dc.subject.other	Metric system	en
dc.subject.other	Polysaccharides	en
dc.subject.other	Probes	en
dc.subject.other	Grading	en
dc.title	New approach in prostate Gleason grading using fluorescence microscopic imaging	en
heal.type	conferenceItem	en
heal.identifier.primary	10.1117/12.831584	en
heal.identifier.secondary	http://dx.doi.org/10.1117/12.831584	en
heal.identifier.secondary	73681J	en
heal.publicationDate	2009	en
heal.abstract	Prostate cancer is a common disease among men with an increasing number of incidences during the last three decades. Histopathological grading of prostate cancer is based on tissue structural abnormalities. Gleason grading system is the gold standard and is based on the organization features of prostatic glands. However, till now there is an uncertainty assign Gleason grade to intermediate stages of the disease, Gleason 3 and Gleason 4. The aim of this study was to explore the possibility of introducing fluorescent probes in this prostate cancer Gleason grading problem. Propidium Iodide with cellular nuclei binding pattern and Alexa 488-WGA with selectivity in polysaccharides with sialic acid residues were finally chosen. Their localisation patterns were assessed using confocal microscopy. Their colocalisation degree was quantified using special developed algorithms of image processing and analysis. The introduced metrics of colocalisation were successfully used to correct classify samples in Gleason 3 and Gleason 4 grades. These metrics were found appropriate to correctly classify 93.10 % of the images into the two classes using the logistic algorithm. The integration of confocal microscopy along with fluorescent probes to pathologist routine, is an approach that cloud lead to prognostic advances. © 2009 SPIE-OSA.	en
heal.journalName	Progress in Biomedical Optics and Imaging - Proceedings of SPIE	en
dc.identifier.doi	10.1117/12.831584	en
dc.identifier.volume	7368	en