Micromanipulation of cells and microparticles using optical fibers

Kotsifaki, DG; Makropoulou, M; Serafetinides, AA

dc.contributor.author	Kotsifaki, DG	en
dc.contributor.author	Makropoulou, M	en
dc.contributor.author	Serafetinides, AA	en
dc.date.accessioned	2014-03-01T02:53:21Z
dc.date.available	2014-03-01T02:53:21Z
dc.date.issued	2011	en
dc.identifier.issn	16057422	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/36259
dc.subject	fiber optical tweezers	en
dc.subject	fluoride glass optical fiber	en
dc.subject	optical tweezers	en
dc.subject.other	Biological objects	en
dc.subject.other	Continuous Wave	en
dc.subject.other	Dielectric microspheres	en
dc.subject.other	Diffraction limited	en
dc.subject.other	Energy output	en
dc.subject.other	Er:YAG lasers	en
dc.subject.other	Fiber trapping	en
dc.subject.other	Fiber-optical	en
dc.subject.other	Fluoride glass	en
dc.subject.other	Focused laser beams	en
dc.subject.other	Focusing lens	en
dc.subject.other	Front end	en
dc.subject.other	He-Ne lasers	en
dc.subject.other	High numerical aperture objective	en
dc.subject.other	High-power	en
dc.subject.other	Micro manipulation	en
dc.subject.other	Micro-objects	en
dc.subject.other	Micro-particles	en
dc.subject.other	Microscope objective	en
dc.subject.other	Microscopic particle	en
dc.subject.other	Optical path	en
dc.subject.other	Optical trap	en
dc.subject.other	Opticaltrapping	en
dc.subject.other	Pulse durations	en
dc.subject.other	Single beam	en
dc.subject.other	Working distances	en
dc.subject.other	Ablation	en
dc.subject.other	Erbium	en
dc.subject.other	Fibers	en
dc.subject.other	Glass	en
dc.subject.other	Laser applications	en
dc.subject.other	Laser tissue interaction	en
dc.subject.other	Lenses	en
dc.subject.other	Micromanipulators	en
dc.subject.other	Microoptics	en
dc.subject.other	Optical communication	en
dc.subject.other	Optical fibers	en
dc.subject.other	Tissue	en
dc.subject.other	Optical tweezers	en
dc.title	Micromanipulation of cells and microparticles using optical fibers	en
heal.type	conferenceItem	en
heal.identifier.primary	10.1117/12.889690	en
heal.identifier.secondary	http://dx.doi.org/10.1117/12.889690	en
heal.identifier.secondary	80921N	en
heal.publicationDate	2011	en
heal.abstract	Optical tweezers is a powerful tool which is used to capture and manipulate microscopic particles such as dielectric microspheres and cells. In the single optical trap the beam is strongly focused to a diffraction limited spot by a high numerical aperture objective. Resently a new version of optical trap was demonstrated with optical fibers. Compared with the common optical tweezers which required high power microscope objective and carefully adjusted optical path, the fiber optical tweezers are compact in size and less expensive. Moreover, they have also a working distance not necessarily close to the objective as for a typical optical tweezers. In this work we present the development of a single beam optical fiber trapping system integrated with an optical fiber ablation system for micromanipulation of biological objects. The fiber trap was formed using a continuous wave He-Ne laser operating at 632.8 nm. The fiber ablation system was formed using a free-running Er:YAG laser operating at 2.94 μm with pulse duration of 80 μm. The ablation beam was coupled into the front end of a fluoride glass optical fiber via a focusing lens of 100 mm and a pinhole of 50 μm. We evaluated the fluoride glass optical fiber as far as attenuation and as far as the spatial distribution of the energy output is concerned. We verified that optical trapping and the micromanipulation of micro objects were easily achieved, by a focused laser beam, emerging from optical fiber inclined at 42 degrees to the sample. © 2011 Copyright Society of Photo-Optical Instrumentation Engineers (SPIE).	en
heal.journalName	Progress in Biomedical Optics and Imaging - Proceedings of SPIE	en
dc.identifier.doi	10.1117/12.889690	en
dc.identifier.volume	8092	en