Red blood cell micromanipulation with elliptical laser beam profile optical tweezers in different osmolarity conditions

Spyratou, E; Makropoulou, M; Serafetinides, AA

dc.contributor.author	Spyratou, E	en
dc.contributor.author	Makropoulou, M	en
dc.contributor.author	Serafetinides, AA	en
dc.date.accessioned	2014-03-01T02:53:26Z
dc.date.available	2014-03-01T02:53:26Z
dc.date.issued	2011	en
dc.identifier.issn	16057422	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/36322
dc.subject	bending modulus	en
dc.subject	line optical tweezers	en
dc.subject	RBC rotation	en
dc.subject	red blood cell folding	en
dc.subject.other	Bending moduli	en
dc.subject.other	Buffer solutions	en
dc.subject.other	Cell surfaces	en
dc.subject.other	Elliptical beams	en
dc.subject.other	Elliptical laser beams	en
dc.subject.other	Folding time	en
dc.subject.other	Isotonic solution	en
dc.subject.other	Laser lights	en
dc.subject.other	Laser power	en
dc.subject.other	line optical tweezers	en
dc.subject.other	Micro manipulation	en
dc.subject.other	Optical force	en
dc.subject.other	Optical trap	en
dc.subject.other	Osmolarity	en
dc.subject.other	RBC rotation	en
dc.subject.other	Red blood cell	en
dc.subject.other	Resultant forces	en
dc.subject.other	Rotational torque	en
dc.subject.other	Shape deformation	en
dc.subject.other	Blood	en
dc.subject.other	Cell membranes	en
dc.subject.other	Elasticity	en
dc.subject.other	Laser applications	en
dc.subject.other	Laser beams	en
dc.subject.other	Laser tissue interaction	en
dc.subject.other	Refractive index	en
dc.subject.other	Tissue	en
dc.subject.other	Optical tweezers	en
dc.title	Red blood cell micromanipulation with elliptical laser beam profile optical tweezers in different osmolarity conditions	en
heal.type	conferenceItem	en
heal.identifier.primary	10.1117/12.889661	en
heal.identifier.secondary	http://dx.doi.org/10.1117/12.889661	en
heal.identifier.secondary	80920T	en
heal.publicationDate	2011	en
heal.abstract	In this work optical tweezers with elliptical beam profiles have been developed in order to examine the effect of optical force on fresh red blood cells (RBC) in isotonic, hypertonic and hypotonic buffer solutions. Considering that the optical force depends essentially on the cell surface and the cytoplasmic refractive index, it is obvious that biochemical modifications associated with different states of the cell will influence its behaviour in the optical trap. Line optical tweezers were used to manipulate simultaneously more than one red blood cell. After we have been manipulated a RBC with an elliptical laser beam profile in an isotonic or hypertonic buffer, we noticed that it rotates by itself when gets trapped by optical tweezers and undergoes folding. Further shape deformations can be observed attributed to the competition between alignment and rotational torque which are transferred by laser light to the cell. In hypotonic buffer RBCs become spherical and do not rotate or fold since the resultant force due to rays emerging from diametrically opposite points of the cell leads to zero torque. Manipulation of fresh red blood cells in isotonic solution by line optical tweezers leads to folding and elongation of trapped RBCs. Membrane elasticity properties such as bending modulus can be estimated by measuring RBC's folding time in function with laser power. © 2011 Copyright Society of Photo-Optical Instrumentation Engineers (SPIE).	en
heal.journalName	Progress in Biomedical Optics and Imaging - Proceedings of SPIE	en
dc.identifier.doi	10.1117/12.889661	en
dc.identifier.volume	8092	en