Combined three-dimensional computer vision and epi-illumination fluorescence imaging system

Gorpas, D; Yova, D; Politopoulos, K

dc.contributor.author	Gorpas, D	en
dc.contributor.author	Yova, D	en
dc.contributor.author	Politopoulos, K	en
dc.date.accessioned	2014-03-01T02:53:35Z
dc.date.available	2014-03-01T02:53:35Z
dc.date.issued	2012	en
dc.identifier.issn	16057422	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/36427
dc.subject	Binocular machine vision	en
dc.subject	Camera calibration	en
dc.subject	Epi-illumination system	en
dc.subject	Excitation source calibration	en
dc.subject	Fluorescence imaging	en
dc.subject	Telecentric lens	en
dc.subject.other	Binocular machine vision	en
dc.subject.other	Camera calibration	en
dc.subject.other	Epi-illumination	en
dc.subject.other	Excitation sources	en
dc.subject.other	Fluorescence imaging	en
dc.subject.other	Binoculars	en
dc.subject.other	Calibration	en
dc.subject.other	Computer vision	en
dc.subject.other	Fluorescence	en
dc.subject.other	Imaging systems	en
dc.subject.other	Inspection	en
dc.subject.other	Inverse problems	en
dc.subject.other	Mean square error	en
dc.subject.other	Medical imaging	en
dc.subject.other	Tissue	en
dc.subject.other	Three dimensional	en
dc.title	Combined three-dimensional computer vision and epi-illumination fluorescence imaging system	en
heal.type	conferenceItem	en
heal.identifier.primary	10.1117/12.907786	en
heal.identifier.secondary	82160N	en
heal.identifier.secondary	http://dx.doi.org/10.1117/12.907786	en
heal.publicationDate	2012	en
heal.abstract	Most of the reported fluorescence imaging methods and systems highlight the need for three-dimensional information of the inspected region surface geometry. The scope of this manuscript is to introduce an epi-illumination fluorescence imaging system, which has been enhanced with a binocular machine vision system for the translation of the inverse problem solution to the global coordinates system. The epi-illumination fluorescence imaging system is consisted of a structured scanning excitation source, which increases the spatial differentiation of the measured data, and a telecentric lens, which increases the angular differentiation. On the other hand, the binocular system is based on the projection of a structured light pattern on the inspected area, for the solution of the correspondence problem between the stereo pair. The functionality of the system has been evaluated on tissue phantoms and calibration objects. The reconstruction accuracy of the fluorophores distribution, as resulted from the root mean square error between the actual distribution and the outcome of the forward solver, was more than 80%. On the other hand, the surface three-dimensional reconstruction of the inspected region presented 0.067±0.004 mm accuracy, as resulted from the mean Euclidean distance between the three-dimensional position of the real world points and those reconstructed. © 2012 SPIE.	en
heal.journalName	Progress in Biomedical Optics and Imaging - Proceedings of SPIE	en
dc.identifier.doi	10.1117/12.907786	en
dc.identifier.volume	8216	en