Electrooptical measurements as process analytical tool for biogas processes: comparative study with Flow Cytometry

Μοσχίδη, Δανάη - Καλλιόπη; Moschidi, Danai - Kalliopi

dc.contributor.author	Μοσχίδη, Δανάη - Καλλιόπη	el
dc.contributor.author	Moschidi, Danai - Kalliopi	en
dc.date.accessioned	2015-06-23T15:02:02Z
dc.date.available	2015-06-23T15:02:02Z
dc.date.issued	2015-06-23
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/40872
dc.identifier.uri	http://dx.doi.org/10.26240/heal.ntua.11420
dc.rights	Default License
dc.subject	Biogas	en
dc.subject	Βιοαέριο	el
dc.subject	Flow cytometry	en
dc.subject	Electrooptical measurements	en
dc.subject	Clostridia	en
dc.subject	Process analytical tool	en
dc.subject	Κυτταρομετρία ροής	el
dc.subject	Ηλεκτροοπτικές μετρήσεις	el
dc.subject	Τεχνολογία ανάλυσης διεργασιών	el
dc.title	Electrooptical measurements as process analytical tool for biogas processes: comparative study with Flow Cytometry	en
heal.type	bachelorThesis
heal.secondaryTitle	Ηλεκτροοπτικές μετρήσεις ως τεχνολογία ανάλυσης διεργασιών για την παραγωγή βιοαερίου: συγκριτική μελέτη με μετρήσεις κυτταρομετρίας ροής	el
heal.classification	Biotechnology	el
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh00007765
heal.language	en
heal.access	campus
heal.recordProvider	ntua	el
heal.publicationDate	2015-03
heal.abstract	Due to rising costs, constant dwindling fossil energy sources, and environmental concerns regarding extraction and use of petroleum and natural gas over the last decades it is considered necessary to shift to the renewable energy sources. Bioenergy and renewable raw materials are two of some economically competitive alternative sustainable energy sources in Germany and in Greece. In Europe, Germany is the biggest biogas production and has the most advanced biogas technology, whereas the biogas production in Greece has not been developed to the extent. A suitable renewable energy source is the biogas which typically refers to a mixture of different gases produced by the breakdown of organic matter in the absence of oxygen. The appropriate conversion and process management technologies need to be optimized to ensure a predefined quality at the end of the manufacturing process. The appropriate measurement program and technologies which design, analyze and control manufacturing processes was defined the Process Analytical Technologies (PAT). In this study, Flow Cytometry and Electrooptical measurements are used as process analytical tools. Flow Cytometry is a laser-based technique used in biotechnology to assess biological and physiological characteristics of several types of cells. Basically, a flow cytometer composed of the fluidics system, the optics system and the electronics system. Different dyes are used, which allows the analysis of specific cellular characteristics at individual cells. There are a variety of fluorescent dyes with different properties which have to be selected according to the purpose of the investigation. In this study, DAPI, BOX, SYTO13 and PI are applied for staining biogas cells.EloTrace is an automatic electrooptical measurement system for detection of parameters of bacterial cells and the most important application is the monitoring of biosynthesis activity, the optimization of fermentation conditions and the process control of fermentation. In this study, the optimization of staining conditions for DAPI was done using a wild-type strain of genus as a model bacterium, Clostridium acetobutylicum ATCC 824. The determination of the variables and their sensitivity for this optimization was performed by Design of Experiments (DoE) and the outcome of two experimental designs was that the optimal conditions for DAPI staining are 37oC as staining temperature, 20 μg/mL as final concentration of DAPI and 30 minutes as staining time. The staining conditions of the three dyes PI, BOX and SYTO13 for biogas samples were based on protocol for Corynebacterium glutamicum cultivations.The application of Flow Cytometry and Electrooptical Measurements for the analysis of biogas samples requires previous sample purification. In this study, five different procedures to prepare the biogas samples were investigated. Regarding to the Flow Cytometry and Electrooptical results, the conclusion for the preparation of biogas samples was that an easier and lower costly treatment had similar results with a more complicated procedure.	en
heal.advisorName	Λυμπεράτος, Γεράσιμος	el
heal.committeeMemberName	Κέκος, Δημήτριος	el
heal.committeeMemberName	Τόπακας, Ευάγγελος	el
heal.academicPublisher	Εθνικό Μετσόβιο Πολυτεχνείο. Σχολή Χημικών Μηχανικών	el
heal.academicPublisherID	ntua
heal.numberOfPages	105 σ.
heal.fullTextAvailability	true