Σύνθεση βιοδραστικών κουμαρινικών αναλόγων και εγκλεισμός τους σε βιοδιασπώμενα PLGA νανοσωματίδια. In vitro αξιολογηση της βιολογικής τους δράσης

Γκιώνης, Λεωνίδας; Gkionis, Leonidas

dc.contributor.author	Γκιώνης, Λεωνίδας	el
dc.contributor.author	Gkionis, Leonidas	en
dc.date.accessioned	2016-04-08T13:27:28Z
dc.date.available	2016-04-08T13:27:28Z
dc.date.issued	2016-04-08
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/42355
dc.identifier.uri	http://dx.doi.org/10.26240/heal.ntua.11879
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ελλάδα	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/gr/	*
dc.subject	Organic chemistry	en
dc.subject	Polymer Science	en
dc.subject	Nanoparticles	en
dc.subject	Cell viability assay	en
dc.subject	Οργανική χημεία	el
dc.subject	Χημεία πολυμερών	el
dc.subject	Κυτοτοξική μελέτη	el
dc.title	Σύνθεση βιοδραστικών κουμαρινικών αναλόγων και εγκλεισμός τους σε βιοδιασπώμενα PLGA νανοσωματίδια. In vitro αξιολογηση της βιολογικής τους δράσης	el
heal.type	bachelorThesis
heal.classification	Chemical engineering	en
heal.language	el
heal.access	free
heal.recordProvider	ntua	el
heal.publicationDate	2015-06-05
heal.abstract	Coumarins are benzopyrone analogues widely distributed in nature. The fused heterocyclic framework of coumarins has served as the prototype scaffold for the synthesis of a wide variety of analogues in order to investigate their biological activity. Coumarins possess a remarkable range of biological properties including antioxidant, anticancer, vasorelaxant, antiviral and anti-inflammatory. In recent years, encapsulation of active ingredients in colloidal nanocarriers has tremendous potential for drug delivery in the pharmaceutical and cosmetics industry. It consists of the entrapment of a drug/compound inside a carrier material, in the aim of enhancing several physicochemical characteristics of the encapsulated substance while obtaining a more efficient and controlled drug delivery as well. This work details the synthesis, structure characterization and evaluation of biological activity of natural and synthetic coumarin analogues. Moreover, in order to tailor the physicochemical characteristics of the compounds (such as low water solubility, potential photosensitivity and air-oxidative decomposition), their encapsulation in PLGA nanoparticles was studied. PLGA was the polymer of choice as it is a biodegradable and biocompatible polymer which has been approved for use in therapeutic devices. The structure characterization of the compounds was performed using NMR spectroscopy whereas their antioxidant and anti-inflammatory activity was investigated using different in vitro assays. Encapsulation in PLGA nanoparticles was achieved via the emulsification-solvent evaporation technique. Size, polydispersity index and ζ-potential of the nanoparticles were measured by Dynamic Light Scattering method whereas the Encapsulation Efficiency (EE) was determined indirectly, using UV-Vis spectroscopy. The cytotoxicity of both the encapsulated and the non-encapsulated compounds was tested by the MTT assay in the human neuroblastoma cell line SK-N-SH.	en
heal.advisorName	Δέτση, Αναστασία	el
heal.committeeMemberName	Παυλάτου, Ευαγγελία	el
heal.committeeMemberName	Βουγιούκα, Σταματίνα	el
heal.academicPublisher	Εθνικό Μετσόβιο Πολυτεχνείο. Σχολή Χημικών Μηχανικών. Τομέας Χημικών Επιστημών (I). Εργαστήριο Οργανικής Χημείας	el
heal.academicPublisherID	ntua
heal.numberOfPages	115 σ.	el
heal.fullTextAvailability	true