Analyzing collateral effects of resistance adaptation in nosocomial pathogens

Ασημακόπουλος, Κωνσταντίνος; Asimakopoulos, Konstantinos

dc.contributor.author	Ασημακόπουλος, Κωνσταντίνος	el
dc.contributor.author	Asimakopoulos, Konstantinos	en
dc.date.accessioned	2016-06-01T09:37:20Z
dc.date.available	2016-06-01T09:37:20Z
dc.date.issued	2016-06-01
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/42579
dc.identifier.uri	http://dx.doi.org/10.26240/heal.ntua.12079
dc.rights	Default License
dc.subject	Αντιβιοτικά	el
dc.subject	Ανθεκτικότητα	el
dc.subject	Εξελικτική προσαρμογή	el
dc.subject	Πολυανθεκτικά βακτήρια	el
dc.subject	Συνεργιστική ανθεκτικότητα/ευαισθησία	el
dc.subject	Multidrug resistant bacteria	en
dc.subject	Antibiotic resistance	en
dc.subject	ESKAPE pathogens	en
dc.subject	Adaptive evolution	en
dc.subject	Collateral resistance/sensitivity	en
dc.title	Analyzing collateral effects of resistance adaptation in nosocomial pathogens	en
heal.type	bachelorThesis
heal.classification	Βιολογικά συστήματα	el
heal.classification	Biological systems	en
heal.language	en
heal.access	free
heal.recordProvider	ntua	el
heal.publicationDate	2015-10-02
heal.abstract	Pathogenic bacteria are an important cause of high mortality rates and high healthcare costs in developing countries. The emergence of resistant species has made the development of new antimicrobial drugs necessary. A first approach to this issue is to understand how bacteria evolve their resistance mechanisms. On this basis, we performed adaptive evolution experiments of the ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baimannii, Pseudomonas aeruginosa and Enterobacter cloacae) organisms using a suite of five antibiotics. The results showed that resistance adaptation is a complex phenomenon that affects major phenotypical features such as the growth rate, which is mainly slowed down. Collateral sensitivity and resistance experiments revealed that adaptation resulted in cross-resistance across different classes of antibiotics in the majority of the cases showing that the mechanisms of resistance are not unique for each drug. The data and the knowledge gathered from the work done in this thesis can be used as a guiding tool during sequence analysis of the adapted strains. We expect that our final findings will shed light on the genetic responses of the ESKAPE pathogens exposed to antimicrobials.	en
heal.advisorName	Τόπακας, Ευάγγελος	el
heal.advisorName	Sommer, Morten	en
heal.committeeMemberName	Τόπακας, Ευάγγελος	el
heal.committeeMemberName	Κέκος, Δημήτριος	el
heal.committeeMemberName	Βουγιούκα, Σταματίνα	el
heal.academicPublisher	Εθνικό Μετσόβιο Πολυτεχνείο. Σχολή Χημικών Μηχανικών	el
heal.academicPublisherID	ntua
heal.numberOfPages	62 σ.	el
heal.fullTextAvailability	true