Effect of bioaugmentation with hydrolytic bacteria and micro aeration on wheat straw biodegradability

Βασιλείου, Σπυρίδων-Αριστοτέλης; Vasileiou, Spyridon-Aristotelis

dc.contributor.author	Βασιλείου, Σπυρίδων-Αριστοτέλης	el
dc.contributor.author	Vasileiou, Spyridon-Aristotelis	en
dc.date.accessioned	2017-01-24T09:27:30Z
dc.date.available	2017-01-24T09:27:30Z
dc.date.issued	2017-01-24
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/44237
dc.rights	Default License
dc.subject	Biofuels	el
dc.subject	Bioenergy	en
dc.subject	Llignocellulosic	en
dc.subject	Bioaugmentation	en
dc.subject	Wheat	en
dc.title	Effect of bioaugmentation with hydrolytic bacteria and micro aeration on wheat straw biodegradability	en
dc.title
heal.type	MasterThesis	en
heal.classification	Environmental	el
heal.language	en
heal.access	free
heal.recordProvider	ntua	el
heal.publicationDate	2016-07-04
heal.abstract	The main purpose of the work was to examine the treatment of lignocellulosic substrate in order to enhance the hydrolysis step in anaerobic digestion (AD) process and subsequently increase the methane yield. In order to enhance hydrolytic step and biodegradability, two different methods were investigated under thermophilic anaerobic conditions, namely Microaeration and Bioaugmentation. Thus, the thesis is divided in two parts. The first part is focused in the microaeration of AD inoculum, compost inoculum and wheat straw through BMP assays. Whereas for the second part, the effect of routine bioaugmentation to cattle manure and wheat straw is investigated through continuous reactors (CSTR) with two different strains of microbes, Clostridium thermocellum (DSMZ 1237) and Melioribacter roseus (DSMZ 23840). Additionally, in order to define the exact methane potential, series of BMP assessments were conducted. In the first experiment 182 batches were prepared in order to probe the competence of facultative anaerobic fungi to hydrolyse wheat straw respiring small amounts of oxygen. The extended assessment of the aforementioned methods is following with main objective to indicate efficient alternative treatment processes for co-digestion of cattle manure and lignocellulosic energy crops such as wheat straw. Compost was used in terms of biological treatment in BMP assays, by means of “methano-compost’’ microflora. Routine bioaugmentation has been reported by many authors as a promising method to interfere in a complex biological system. In the thesis highly efficient hydrolytic bacteria were injected in order to excrete degrading enzymes and disrupt the recalcitrant part of wheat straw, lignin. More specifically, these strains were Clostridium thermocellum (DSMZ 1237) and Melioribacter roseus (DSMZ 23840). Both strains require for their growth anaerobic thermophilic conditions, except from M. roseus which is facultative anaerobic. The implementation and main objective of bioaugmentation was the long term infection and colonization in the new environment, avoiding the wash out of injected microorganisms. Nevertheless, the main aim was to obtain a new eco-system slightly adjusted by the new hydrolytic strains in aid of us.	en
heal.advisorName	Λυμπεράτος, Γεράσιμος	el
heal.committeeMemberName	Βλυσίδης, Απόστολος	el
heal.academicPublisher	Εθνικό Μετσόβιο Πολυτεχνείο. Σχολή Χημικών Μηχανικών	el
heal.academicPublisherID	ntua
heal.fullTextAvailability	true