Παραγωγή βιοαιθανόλης από τη ζύμη Trichosporon fermentans

Ζουλιάτης, Αλέξανδρος; Zouliatis, Alexandros

dc.contributor.author	Ζουλιάτης, Αλέξανδρος	el
dc.contributor.author	Zouliatis, Alexandros	en
dc.date.accessioned	2017-02-15T10:21:34Z
dc.date.available	2017-02-15T10:21:34Z
dc.date.issued	2017-02-15
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/44362
dc.identifier.uri	http://dx.doi.org/10.26240/heal.ntua.13615
dc.rights	Default License
dc.subject	Βιοκαύσιμα	el
dc.subject	Παραγωγή βιοαιθανόλης	el
dc.subject	Ζύμη	el
dc.subject	Προκατεργασμένο άχυρο σίτου	el
dc.subject	Βυθισμένες καλλιέργειες	el
dc.subject	Biofuels	en
dc.subject	Bioethanol	en
dc.subject	Trichosporon fermentans	en
dc.subject	Fusarium oxysporum	en
dc.subject	Pretreated wheat straw	en
dc.title	Παραγωγή βιοαιθανόλης από τη ζύμη Trichosporon fermentans	el
heal.type	bachelorThesis
heal.classification	Βιοχημική τεχνολογία και μηχανική-βιοτεχνολογία	el
heal.classificationURI	http://data.seab.gr/concepts/7976b2f9d3bd98af08dc6a9a4f2e947144d26b5d
heal.language	el
heal.access	free
heal.recordProvider	ntua	el
heal.publicationDate	2016-10-07
heal.abstract	Στην παρούσα διπλωματική εργασία μελετήθηκε η δυνατότητα του μικροοργανισμού Trichosporon fermentans ως προς την παραγωγή αιθανόλης. Η παραγωγή μελετήθηκε αρχικά σε μονοκαλλιέργεια, προκειμένου να καθοριστούν οι βέλτιστες συνθήκες απόδοσης σε αιθανόλη, όπως π.χ. pH, πηγές αζώτου, πηγές άνθρακα κ.α. και στη συνέχεια σε συγκαλλιέργεια με το μύκητα Fusarium oxysporum προκειμένου να διερευνηθεί μία περαιτέρω αύξηση της απόδοσης αυτής	el
heal.abstract	The goal of this thesis work was the study of ethanol production from the oleaginous yeast Trichosporon fermantans, an organism thoroughly studied for its ability to accumulate lipids but not for its ability to ferment substrates to ethanol. To this end, different experiments were carried out either on simple or synthetic growth media. At first, the fermentation process was studied on simple media to determine the effects of nitrogen and carbon source and that of the initial sugar concentration, the favorable initial pH region and the tolerance showed by the yeast to ethanol. The optimal nitrogen source was proved to be a mixture of yeast extract and peptone at a concentration of 10 g/L and a ratio of 1:1. T. fermentans presented a really good adaptability at environments slightly acidic to slightly alkaline, with the optimum pH for ethanol production being 6.0. Furthermore, it was determined that at initial glucose concentrations in the region of 25 – 100 g/L the yeast was able to perform well, achieving yields of up to 0.461 g/g, whereas substrate inhibition was displayed at initial concentrations of 200 g/L and above. When tested on substrates other than glucose, T. fermentans was able to satisfactorily utilize only fructose achieving a yield of 0.402 g/g and not lactose, xylose or arabinose, a phenomenon attributed to the aeration conditions, since it has been shown by other investigators that the yeast has the ability to assimilate pentozes in fully oxygenated environments. When tested under ethanol stress, the microorganism was able to successfully ferment sugars for initial concentration of ethanol up to 3% w/v, whereas at 7% w/v its metabolic activity seems to completely stop displaying no ethanol production at all. In the second part of this thesis work, the yeast was tested on synthetic media, namely on High Hemicellulose Pretreated Wheat Straw of 23% Dry Matter ( HHPWS 23% DM). The HHPWS was liquefied with the addition of a mixture of thermoenzymes in an enzymatic liquefaction reactor and fermented for 5 days. This resulted in a final concentration of 21.63 g/kg of ethanol produced and a yield of 29% calculated on the total sugars hydrolyzed. To further enhance the ethanol production and the yield achieved, Fusarium oxysporum cells were added to the culture, a microorganism with the ability of hydrolyzing cellulose and hemicellulose, resulting in the development of a Simultaneous Saccharification and Co-Fermentation system (SSCF). The final concentration of ethanol was improved by 33.5% percent reaching a final concentration of 28.87 g/kg of ethanol on the fifth day, but evaluating the system on the total amount of sugars contained in the media the total yield was a mere 17.2%.	en
heal.advisorName	Τόπακας, Ευάγγελος	el
heal.committeeMemberName	Κέκος, Δημήτριος	el
heal.committeeMemberName	Κοκόσης, Αντώνιος	el
heal.academicPublisher	Εθνικό Μετσόβιο Πολυτεχνείο. Σχολή Χημικών Μηχανικών	el
heal.academicPublisherID	ntua
heal.numberOfPages	116 σ.	el
heal.fullTextAvailability	true