Modulation on enzyme activity - case study for Lytic polysaccharide monooxygenases

Διαμαντόπουλος, Βασίλειος; Diamantopoulos, Vasileios

dc.contributor.author	Διαμαντόπουλος, Βασίλειος	el
dc.contributor.author	Diamantopoulos, Vasileios	en
dc.date.accessioned	2018-10-08T09:35:49Z
dc.date.available	2018-10-08T09:35:49Z
dc.date.issued	2018-10-08
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/47736
dc.identifier.uri	http://dx.doi.org/10.26240/heal.ntua.15002
dc.rights	Default License
dc.subject	Cellulose	en
dc.subject	Cellulases	en
dc.subject	LPMOs	en
dc.subject	Tannis	en
dc.subject	Persimmon	en
dc.title	Modulation on enzyme activity - case study for Lytic polysaccharide monooxygenases	en
heal.type	bachelorThesis
heal.generalDescription	Η εργασία εκπονήθηκε μέσω του προγράμματος Erasmus στο πανεπιστήμιο του Chalmers στο Gothenburg της Σουηδίας και συγκεκριμένα στο εργαστήριο Industrial Biotechnology την περίοδο Φεβρουάριο με Ιούλιο του 2017.	el
heal.classification	Ενζυμική βιοτεχνολογία	el
heal.classificationURI	http://data.seab.gr/concepts/fe3c8c2ca5d49c76e9b08c232ebf31b4f8b2d74e
heal.language	en
heal.access	free
heal.recordProvider	ntua	el
heal.publicationDate	2018-02-23
heal.abstract	Lignocellulosic biomass is one of the most promising alternative resources for production of biofuels and added valued products. Conversion of lignocellulosic biomass into fermentable sugars is a very important step in lignocellulosic ethanol production. Saccharification of biomass using enzymes, called cellulases, is an attractive process but still, there is a great deal of scope for research to improve the enzymatic conversion efficiency. The efficiency of the saccharification is based on advances in the enzymatic cocktail ingredients, of which one of the most significant is the recent inclusion of Lytic polysaccharide monooxygenases (LPMOs). LPMOs are of increased biotechnological and scientific interest due to their implication in lignocellulosic biomass decomposition as they are the first enzymes that act to the crystalline cellulose making the substrate easier accessible to the other enzymes for the complete decomposition. Through the years, after the discovery of LPMOs, information about them that they have been published are about their classification, their structure, the identification of cooper as the metal in their active site, their mechanism, and what activates them. In this project, the effect of a plant extract that comes for a persimmon fruit to fungal LPMOs and to beta-glycosidase was investigated. The overall aim was the search of a modulator, which can be either an inhibitor or an activator, with the plant extract as a source for modulators. For this purpose, saccharification experiments were done using cellulose as substrate and LPMO and beta-glucosidase as enzymes. Also, fractionation of the plant extract took place using liquid-liquid extraction. With the saccharification experiments the effect of the plant extract and the fractions of it were tested on these two enzymes. Last the effect on tannins on these two enzymes was tested using dilutions of tannic acid in the saccharification experiments. It is concluded that the plant extract and the tannic acid has an inhibitory effect to the LPMOs and beta-glucosidase, with higher effect to LPMOs.	en
heal.sponsor	The project was funded by the Swedish Energy Agency (Energimyndigheten) under the grant № 41259-1. Enzymes were provided by Novozymes A/S.	en
heal.advisorName	Τόπακας, Ευάγγελος	el
heal.committeeMemberName	Κέκκος, Δημήτριος	el
heal.committeeMemberName	Γρηγοροπούλου, Ελένη	el
heal.academicPublisher	Εθνικό Μετσόβιο Πολυτεχνείο. Σχολή Χημικών Μηχανικών. Τομέας Σύνθεσης και Ανάπτυξης Βιομηχανικών Διαδικασιών (IV)	el
heal.academicPublisherID	ntua
heal.numberOfPages	90 σ.
heal.fullTextAvailability	true