Αξιοποίηση ενός οξειδωτικού ενζύμου τύπου λακκάσης
για την ανίχνευση νευροδιαβιβαστών από ηλεκτροχημικό βιοαισθητήρα

Κιάφη, Σωτηρία; Kiafi, Sotiria

dc.contributor.author	Κιάφη, Σωτηρία
dc.contributor.author	Kiafi, Sotiria	en
dc.date.accessioned	2019-11-26T11:50:27Z
dc.date.available	2019-11-26T11:50:27Z
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/49502
dc.identifier.uri	http://dx.doi.org/10.26240/heal.ntua.17200
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ελλάδα	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/gr/	*
dc.subject	Λακκάση	el
dc.subject	Επινεφρίνη	el
dc.subject	Ηλεκτροχημεία	el
dc.subject	Φυσικοχημεία	el
dc.subject	Βιοηλεκτροχημεία	el
dc.subject	Laccase	en
dc.subject	Epinephrine	en
dc.subject	LA-FTacV	en
dc.subject	bioelectrochemistry	en
dc.subject	FTacV	en
dc.subject	Biosensor	en
dc.title	Αξιοποίηση ενός οξειδωτικού ενζύμου τύπου λακκάσης για την ανίχνευση νευροδιαβιβαστών από ηλεκτροχημικό βιοαισθητήρα	el
heal.type	bachelorThesis
heal.classification	Ηλεκτροχημεία	el
heal.classification	Φυσικοχημεία	el
heal.language	el
heal.access	free
heal.recordProvider	ntua	el
heal.publicationDate	2019-09-26
heal.abstract	Μέσω της μεθόδου της LA-FTacV αξιοποιείται οξειδωτικό ένζυμο με ενεργότητα λακκάσης προκειμένου να ανιχνευτούν νευροδιαβιβαστικές ουσίες ελεύθερες σε διάλυμα. Πιο συγκεκριμένα, γίνεται ανίχνευση της επινεφρίνης, ή κοινώς αδρεναλίνης, αφού πρόκειται για ουσία ενδεικτική εξέλιξης ασθενειών και χρήσιμη για μελέτη δράσης φαρμάκων. Για την ανίχνευση γίνεται χρήση τόσο κυκλικής βολταμμετρίας, αλλά και μιας παραλλαγής της, της LA-FTacV, η οποία αποτελεί πολύτιμο εργαλείο για μελέτη ουσιών σε τόσο μικρές ποσότητες.	el
heal.abstract	In this thesis, the redox behavior of epinephrine, as well as the redox properties of a laccase type enzyme, being isolated by the thermophile fungus Myceliophthora Thermophila,are being examined.The study of these compounds is conducted with the use of cyclic voltammetry (CV) and large amplitude ac voltammetry (LA-FTacV). The two substances are being studied individually, as well as in conjunction, with the larger goal of this study being the pinpointing of ideal experimental conditions for identifying the epinephrinemolecule, with the usage of the enzyme for biosensor applications.The LA-FTacV method being used in this study is free of the problems that come with classic cyclic voltammetry. Other than using the classic dc enforcementwhich is typical for cyclic voltammetry, in this case a sinusoidal enforcement is also being used on the potential. The signal is being processed with Fourier transformation and after that, the capacity currents and the noiseare eliminated. This method also has the potential for verifying other thermodynamic measurements, such as theapparentelectrochemical potential Eo’.The enzyme, immobilized by Nafion electrolyte, and the epinephrine are being studied individually on three different electrodes:glassy carbon, Au and pyrolytic carbon. Both these substances behave better on the pyrolytic carbon electrode, taking in consideration different conditions in amplitude and frequency. As a result, the combined system is being studied only on that electrode. The effect of the temperature is being examined in a spectrum between 25 and 50 oC, with a step of 5. For the enzyme, the apparent electrochemical potential Eo’was determined for varied values of temperature. The redox behavior of epinephrine is being examined and graphed through the molecule’s signal in different conditions of amplitude and frequency and the direct detection from the working electrode is also being examined. Different values of pH are also being takeninto consideration, so that the optimal conditions for the experiment are being meet, these being 1 Hz, pH 4anda temperature of 30 oC. The results of this method are being compared with papers for epinephrine detectionand quantificationfrom other methods, with the result being benefits of LA-FTacV getting highlighted	en
heal.advisorName	Καραντώνης, Αντώνης
heal.committeeMemberName	Τσόπελας, Φώτιος
heal.committeeMemberName	Τόπακας, Ευάγγελος
heal.academicPublisher	Εθνικό Μετσόβιο Πολυτεχνείο. Σχολή Χημικών Μηχανικών. Τομέας Επιστήμης και Τεχνικής των Υλικών (ΙΙΙ)	el
heal.academicPublisherID	ntua
heal.numberOfPages	109 σ.
heal.fullTextAvailability	false