Μεθοδολογία και λειτουργία κάμερας ανιχνευτή φωτονίων για βιοϊατρικές εφαρμογές

Panteleakis, Philip; Παντελεάκης Φίλιππος

dc.contributor.author	Panteleakis, Philip	en
dc.contributor.author	Παντελεάκης Φίλιππος	el
dc.date.accessioned	2022-11-10T09:07:19Z
dc.date.available	2022-11-10T09:07:19Z
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/56113
dc.identifier.uri	http://dx.doi.org/10.26240/heal.ntua.23811
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ελλάδα	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/gr/	*
dc.subject	CCD cameras	en
dc.subject	Απεικόνιση ασβεστίου	el
dc.subject	Φθορίζουσα απεικόνιση	el
dc.subject	CCD κάμερες	el
dc.subject	Calcium imaging	en
dc.subject	Fluorescence imaging	en
dc.title	Μεθοδολογία και λειτουργία κάμερας ανιχνευτή φωτονίων για βιοϊατρικές εφαρμογές	el
dc.contributor.department	Ηλεκτρονικών Αισθητηρίων (NTUA) & Φυσιολογείον (NKUA)	el
heal.type	masterThesis
heal.secondaryTitle	Methodology and operation of a photon detector camera for biomedical applications	en
heal.classification	Sensing technology	en
heal.language	el
heal.language	en
heal.access	free
heal.recordProvider	ntua	el
heal.publicationDate	2022-09-19
heal.abstract	This postgraduate thesis explores the functional principles and practical methodology of CCD cameras used in the imaging technique of wide-field fluorescence microscopy. To this end, the basic theory behind the photodetecting ability of CCDs and the fluorescence emission of fluorophores is presented together with the general concept of an imaging system, its instrumentation and its coordinated operation. Nerve circuit functional imaging experiments were conducted in-vivo on Drosophila Melanogaster fruit flies, aiming to map their auditory system spectrum. The basic principles of neurophysiology and the anatomy of Drosophila’s auditory system provide a theoretical framework for this work. Specifically, responses of the Johnstone’s organ stretch-receptor axons were recorded across a chosen range of sound frequencies. Targetted expression of a GECI in Drosophila’s neural system and action potential calcium dynamics allowed quantitive measurements of Drosophila’s auditory signals by means of calcium imaging. Cross-breeds of UAS-GCaMP and GAL4-elav Drosophila mutants were cultured and used in these experiments. Effective imaging of fluorescence emission was carried out by using a TILLvisION imaging system comprised of a PCO QE Sensicam CCD, a Nikon Eclipse E600 FN epifluorescence microscope, a TILL Photonics Polychrome V monochromator, a TILLvisION Control Unit and a TILLvisION software. Diagrams of fluorescence signals deriving from chosen ROIs allowed the analysis of fluorescence emissions. The results were compared with respective electrophysiology measurements. The comparison shows that Sensicam QE CCD has the potential of detecting Ca+2 dynamics at the level of multiple neurons (compound action potentials) and it can be used for similar research.	en
heal.advisorName	Hristoforou, Evangelos	en
heal.committeeMemberName	Hristoforou, Evangelos	en
heal.committeeMemberName	Christos, Consulas	en
heal.committeeMemberName	Ktena, Afrodith	en
heal.academicPublisher	Εθνικό Μετσόβιο Πολυτεχνείο. Σχολή Εφαρμοσμένων Μαθηματικών και Φυσικών Επιστημών. Τομέας Φυσικής	el
heal.academicPublisherID	ntua
heal.numberOfPages	121 σ.	el
heal.fullTextAvailability	false