Μεθοδολογία και λειτουργία κάμερας ανιχνευτή φωτονίων για βιοϊατρικές εφαρμογές

DSpace/Manakin Repository

Show simple item record

dc.contributor.author Panteleakis, Philip en
dc.contributor.author Παντελεάκης Φίλιππος el
dc.date.accessioned 2022-11-10T09:07:19Z
dc.date.available 2022-11-10T09:07:19Z
dc.identifier.uri https://dspace.lib.ntua.gr/xmlui/handle/123456789/56113
dc.identifier.uri http://dx.doi.org/10.26240/heal.ntua.23811
dc.rights Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ελλάδα *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/gr/ *
dc.subject CCD cameras en
dc.subject Απεικόνιση ασβεστίου el
dc.subject Φθορίζουσα απεικόνιση el
dc.subject CCD κάμερες el
dc.subject Calcium imaging en
dc.subject Fluorescence imaging en
dc.title Μεθοδολογία και λειτουργία κάμερας ανιχνευτή φωτονίων για βιοϊατρικές εφαρμογές el
dc.contributor.department Ηλεκτρονικών Αισθητηρίων (NTUA) & Φυσιολογείον (NKUA) el
heal.type masterThesis
heal.secondaryTitle Methodology and operation of a photon detector camera for biomedical applications en
heal.classification Sensing technology en
heal.language el
heal.language en
heal.access free
heal.recordProvider ntua el
heal.publicationDate 2022-09-19
heal.abstract This postgraduate thesis explores the functional principles and practical methodology of CCD cameras used in the imaging technique of wide-field fluorescence microscopy. To this end, the basic theory behind the photodetecting ability of CCDs and the fluorescence emission of fluorophores is presented together with the general concept of an imaging system, its instrumentation and its coordinated operation. Nerve circuit functional imaging experiments were conducted in-vivo on Drosophila Melanogaster fruit flies, aiming to map their auditory system spectrum. The basic principles of neurophysiology and the anatomy of Drosophila’s auditory system provide a theoretical framework for this work. Specifically, responses of the Johnstone’s organ stretch-receptor axons were recorded across a chosen range of sound frequencies. Targetted expression of a GECI in Drosophila’s neural system and action potential calcium dynamics allowed quantitive measurements of Drosophila’s auditory signals by means of calcium imaging. Cross-breeds of UAS-GCaMP and GAL4-elav Drosophila mutants were cultured and used in these experiments. Effective imaging of fluorescence emission was carried out by using a TILLvisION imaging system comprised of a PCO QE Sensicam CCD, a Nikon Eclipse E600 FN epifluorescence microscope, a TILL Photonics Polychrome V monochromator, a TILLvisION Control Unit and a TILLvisION software. Diagrams of fluorescence signals deriving from chosen ROIs allowed the analysis of fluorescence emissions. The results were compared with respective electrophysiology measurements. The comparison shows that Sensicam QE CCD has the potential of detecting Ca+2 dynamics at the level of multiple neurons (compound action potentials) and it can be used for similar research. en
heal.advisorName Hristoforou, Evangelos en
heal.committeeMemberName Hristoforou, Evangelos en
heal.committeeMemberName Christos, Consulas en
heal.committeeMemberName Ktena, Afrodith en
heal.academicPublisher Εθνικό Μετσόβιο Πολυτεχνείο. Σχολή Εφαρμοσμένων Μαθηματικών και Φυσικών Επιστημών. Τομέας Φυσικής el
heal.academicPublisherID ntua
heal.numberOfPages 121 σ. el
heal.fullTextAvailability false

Files in this item

The following license files are associated with this item:

This item appears in the following Collection(s)

Show simple item record

Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ελλάδα Except where otherwise noted, this item's license is described as Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ελλάδα