Transcriptomic, signalling and secretome analysis for biomarker discovery in ageing and senescence

Σκέα, Διονυσία; Skea, Dionysia

dc.contributor.author	Σκέα, Διονυσία	el
dc.contributor.author	Skea, Dionysia	en
dc.date.accessioned	2023-09-04T09:35:27Z
dc.date.available	2023-09-04T09:35:27Z
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/57947
dc.identifier.uri	http://dx.doi.org/10.26240/heal.ntua.25644
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ελλάδα	*
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ελλάδα	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/gr/	*
dc.subject	Senescence	en
dc.subject	Aging	en
dc.subject	Transcriptome	en
dc.subject	Secretome	en
dc.subject	Biomarker	en
dc.subject	Γήρανση	el
dc.subject	Μεταγράφομα	el
dc.subject	Βιοδείκτης	el
dc.subject	Πρωτεϊνικές εκκρίσεις	el
dc.title	Transcriptomic, signalling and secretome analysis for biomarker discovery in ageing and senescence	en
dc.title	Ανάλυση σηματοδοτικών μονοπατιών, πρωτεϊνικών εκκρίσεων και μεταγραφόματος για την ανεύρεση βιοδεικτών κατά την γήρανση	el
dc.contributor.department	Τομέας Μηχανολογικών Κατασκευών & Αυτομάτου Ελέγχου	el
heal.type	doctoralThesis
heal.classification	Systems Biology	en
heal.language	en
heal.access	campus
heal.recordProvider	ntua	el
heal.publicationDate	2023-06-27
heal.abstract	Cellular senescence is described as an irreversible cell cycle arrest induced in response to various stresses. Senescent cells are characterised by heterogeneous signalling alterations, complex secretory phenotype, known as senescence-associated secretory phenotype (SASP), and diverse transcriptomic profile. With the aim to investigate senescence heterogeneity and identify conserved transcriptomic alterations and universal senescence markers, we performed RNA-seq and multiplex analysis in proteasome inhibition-induced and stress-induced premature senescence models of HFL1 and BJ human fibroblasts. Our data revealed diverse transcriptomic signatures, but also, 231 common differentially expressed genes related to cell division and extracellular matrix (ECM) remodelling, and enriched pathways that remained conserved among the different models, with senescence onset. Moreover, we identified a subset of proteins inducing cell cycle arrest and promoting a pro-inflammatory environment in premature senescence models which showed consistent results when validated in their respective replicative senescent models. Finally, we propose an intracellular protein panel consisting of p21, p-c-JUN, BCL-xL and survivin, and an extracellular panel consisting of IL-8, GM-CSF, GDF-15, MIF and GROa. Simultaneous assessment of these markers will enable the easier identification of senescent cells and monitor the efficacy of potential senotherapeutic approaches.	en
heal.advisorName	Αλεξόπουλος, Λεωνίδας	el
heal.advisorName	Alexopoulos, Leonidas G	en
heal.advisorName	Chatziioannou, Aristotelis
heal.advisorName	Chronis, Nikos
heal.committeeMemberName	Manopoulos, Christos
heal.committeeMemberName	Chondrogianni, Niki
heal.committeeMemberName	KOLLIA, PANAGOULA
heal.committeeMemberName	Kletsas, Dimitris
heal.academicPublisher	Εθνικό Μετσόβιο Πολυτεχνείο. Σχολή Μηχανολόγων Μηχανικών	el
heal.academicPublisherID	ntua
heal.numberOfPages	178
heal.fullTextAvailability	false