Extracts from Chlorella vulgaris Protect Mesenchymal Stromal Cells from Oxidative Stress Induced by Hydrogen Peroxide

Savvidou, Maria; Georgiopoulou, Ioulia; Antoniou, Nasia; Tzima, Soultana; Kontou, Maria; Louli, Vasiliki; Fatouros, Chronis; Magoulas, Kostis; Kolisis, Fragiskos

dc.contributor.author	Savvidou, Maria
dc.contributor.author	Georgiopoulou, Ioulia
dc.contributor.author	Antoniou, Nasia
dc.contributor.author	Tzima, Soultana
dc.contributor.author	Kontou, Maria
dc.contributor.author	Louli, Vasiliki
dc.contributor.author	Fatouros, Chronis
dc.contributor.author	Magoulas, Kostis
dc.contributor.author	Kolisis, Fragiskos
dc.date.accessioned	2023-10-11T13:36:50Z
dc.date.available	2023-10-11T13:36:50Z
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/58152
dc.identifier.uri	http://dx.doi.org/10.26240/heal.ntua.25848
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ελλάδα	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/gr/	*
dc.subject	microalgae	en
dc.subject	oxidative stress	en
dc.subject	bioactive components	en
dc.subject	Chlorella vulgaris	en
dc.subject	mesenchymal cells	en
dc.subject	supercritical fluid extraction	en
dc.subject	antioxidant activity	en
dc.subject	cell viability	en
dc.title	Extracts from Chlorella vulgaris Protect Mesenchymal Stromal Cells from Oxidative Stress Induced by Hydrogen Peroxide	en
heal.type	journalArticle
heal.classification	Chemical Engineering	en
heal.classification	Biotechnology	en
heal.language	en
heal.access	free
heal.recordProvider	ntua	el
heal.publicationDate	2023-01-12
heal.bibliographicCitation	Savvidou, M. G., Georgiopoulou, I., Antoniou, N., Tzima, S., Kontou, M., Louli, V., Fatouros,C., Magoulas, K., & Kolisis, F. N., Extracts from Chlorella vulgaris Protect Mesenchymal Stromal Cells from Oxidative Stress Induced by Hydrogen Peroxide. Plants, 12(2), 361, MDPI, 2023.	en
heal.abstract	Microalgae as unicellular eukaryotic organisms demonstrate several advantages for biotechnological and biological applications. Natural derived microalgae products demand has increased in food, cosmetic and nutraceutical applications lately. The natural antioxidants have been used for attenuation of mitochondrial cell damage caused by oxidative stress. This study evaluates the in vitro protective effect of Chlorella vulgaris bioactive extracts against oxidative stress in human mesenchymal stromal/stem cells (MSCs). The classical solid-liquid and the supercritical extraction, using biomass of commercially available and laboratory cultivated C. vulgaris, are employed. Oxidative stress induced by 300 μM H2O2 reduces cell viability of MSCs. The addition of C. vulgaris extracts, with increased protein content compared to carbohydrates, to H2O2 treated MSCs counteracted the oxidative stress, reducing reactive oxygen species levels without affecting MSC proliferation. The supercritical extraction was the most efficient extraction method for carotenoids resulting in enhanced antioxidant activity. Pre-treatment of MSCs with C. vulgaris extracts mitigates the oxidative damage ensued by H2O2. Initial proteomic analysis of secretome from licensed (TNFα-activated) MSCs treated with algal extracts reveals a signature of differentially regulated proteins that fall into clinically relevant pathways such as inflammatory signaling. The enhanced antioxidative and possibly anti-inflammatory capacity could be explored in the context of future cell therapies.	en
heal.sponsor	This research has been co-financed by the European Regional Development Fund of the European Union and Greek National Funds through the Operational Program Competitiveness, Entrepreneurship and Innovation, under the call RESEARCH–CREATE–INNOVATE (project code: BIONAMA T1EDK-04223).	en
heal.publisher	MDPI	en
heal.journalName	Plants	en
heal.journalType	peer-reviewed
heal.fullTextAvailability	false
dc.identifier.doi	https://doi.org/10.3390/plants12020361	el