Comparative Study of Conventional, Microwave-Assisted and Supercritical Fluid Extraction of Bioactive Compounds from Microalgae: The Case of Scenedesmus obliquus

Georgiopoulou, Ioulia; Louli, Vasiliki; Magoulas, Kostis

dc.contributor.author	Georgiopoulou, Ioulia
dc.contributor.author	Louli, Vasiliki
dc.contributor.author	Magoulas, Kostis
dc.date.accessioned	2023-10-11T13:57:38Z
dc.date.available	2023-10-11T13:57:38Z
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/58156
dc.identifier.uri	http://dx.doi.org/10.26240/heal.ntua.25852
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ελλάδα	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/gr/	*
dc.subject	microalgae	en
dc.subject	solid–liquid extraction	en
dc.subject	microwave-assisted extraction	en
dc.subject	supercritical fluid extraction	en
dc.subject	phenolic compounds	en
dc.subject	carotenoids	en
dc.subject	chlorophylls	en
dc.subject	antioxidant activity	en
dc.title	Comparative Study of Conventional, Microwave-Assisted and Supercritical Fluid Extraction of Bioactive Compounds from Microalgae: The Case of Scenedesmus obliquus	en
heal.type	journalArticle
heal.language	en
heal.access	free
heal.recordProvider	ntua	el
heal.publicationDate	2023-05-04
heal.bibliographicCitation	Georgiopoulou, I., Louli, V., & Magoulas, K., Comparative Study of Conventional, Microwave-Assisted and Supercritical Fluid Extraction of Bioactive Compounds from Microalgae: The Case of Scenedesmus obliquus. Separations, 10(5), 290, MDPI, 2023.	en
heal.abstract	The recovery of bioactive products with green processes is a critical topic for the research and industry fields. In this work, the application of solid–liquid (SLE), microwave-assisted extraction (MAE) with aq. ethanol 90% v/v and supercritical fluid extraction (SFE) with CO2 for the recovery of biocomponents from Scenedesmus obliquus is studied. The effects examined were temperature (30–60 °C), time (6–24 h), and solvent-to-biomass ratio (20–90 mLsolv/gbiom) for SLE, temperature (40–60 °C), time (5–25 min), solvent-to-biomass ratio (20–90 mLsolv/gbiom), and microwave power (300–800 W) for MAE, and temperature (40–60 °C), pressure (110–250 bar), solvent flow rate (20–40 gsolv/min), and cosolvent presence (0, 10% w/w ethanol) for SFE in relation to the extract’s yield, phenolic, chlorophyll, carotenoid content, and antioxidant activity. The optimum extraction conditions determined were 30 °C, 24 h, and 90 mLsolv/gbiom for SLE, 60 °C, 5 min, 90 mLsolv/gbiom, and 300 W for MAE, and 60 °C, 250 bar, and 40 gsolv/min for SFE. Additionally, a kinetic SFE study was conducted and the obtained results were satisfactorily correlated using Sovová’s model. The comparison between the methods proved MAE’s efficiency in all terms compared to SLE. Moreover, SFE was accompanied with the lowest yield and chlorophyll content, yet led to an increased carotenoid content and improved antioxidant activity. Finally, the cosolvent addition significantly improved SFE’s yield and led to the most superior extract.	en
heal.sponsor	The implementation of the doctoral thesis of the author Ioulia Georgiopoulou was co-financed by Greece and the European Union (European Social Fund-ESF) through the Operational Program “Human Resources Development, Education and Lifelong Learning” in the context of the Act “Enhancing Human Resources Research Potential by undertaking a Doctoral Research” Sub-action 2: IKY Scholarship Program for PhD candidates in Greek Universities.	en
heal.publisher	MDPI	en
heal.journalName	Separations	en
heal.journalType	peer-reviewed
heal.fullTextAvailability	false
dc.identifier.doi	https://doi.org/10.3390/separations10050290	el