Differential gene expression in human fibroblasts simultaneously exposed to ionizing radiation and simulated microgravity

Malatesta, Polina; Μαλατέστα, Πολίνα

dc.contributor.author	Malatesta, Polina	el
dc.contributor.author	Μαλατέστα, Πολίνα	en
dc.date.accessioned	2024-09-19T08:18:53Z
dc.date.available	2024-09-19T08:18:53Z
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/60233
dc.identifier.uri	http://dx.doi.org/10.26240/heal.ntua.27929
dc.rights	Default License
dc.subject	Μικροβαρύτητα	el
dc.subject	Διαστημική πτήση	el
dc.subject	Κοσμική ακτινοβολία	el
dc.subject	Διαφορικά Εκφρασμένα Γονίδια	el
dc.subject	Δίκτυα γονιδίων	el
dc.subject	Microgravity	en
dc.subject	Space flight	en
dc.subject	Cosmic radiation	en
dc.subject	Differentially Expressed Genes	en
dc.subject	Gene networks	en
dc.title	Differential gene expression in human fibroblasts simultaneously exposed to ionizing radiation and simulated microgravity	en
dc.title	Διαφορική γονιδιακή έκφραση σε ανθρώπινους ινοβλάστες εκτεθειμένους ταυτοχρόνων σε ιοντίζουσα ακτινοβολία και προσομοιωμένη μικροβαρύτητα	el
heal.type	bachelorThesis
heal.classification	Βιοπληροφορική	el
heal.access	free
heal.recordProvider	ntua	el
heal.publicationDate	2024-06-25
heal.abstract	During future space missions, astronauts will be exposed to cosmic radiation and microgravity (μG), known to be health risk factors. To examine the differentially expressed genes (DEGs) and their prevalent biological processes and pathways as a response to these two risk factors simultaneously, 1BR-hTERT human fibroblast cells were cultured under 1 gravity (1G) or simulated μG for 48 hrs in total and collected 0 (sham-irradiated), 3 or 24 hrs after 1 Gy of X-ray or Carbon-ion (C-ion) irradiation. A three-dimensional clinostat was used for the simulation of μG and the simultaneous radiation exposure of the samples. RNA-seq method was used to produce lists of differentially expressed genes between different environmental conditions. Over-representation analyses were performed and the enriched biological pathways and targeting transcription factors were identified. Comparing sham-irradiated cells under simulated μG and 1G conditions, terms related to response to oxygen levels and muscle contraction were identified. After irradiation with X-rays or C-ions under 1G, identified DEGs were found to be involved in DNA damage repair, signal transduction by p53 class mediator, cell cycle arrest and apoptosis pathways. The same enriched pathways emerged when cells were irradiated under simulated μG condition. Nevertheless, the combined effect attenuated the transcriptional response to irradiation which may pose a subtle risk in space flights.	en
heal.advisorName	Γεωργακίλας, Αλέξανδρος	el
heal.committeeMemberName	Γεωργακίλας, Αλέξανδρος	el
heal.committeeMemberName	Διακάκη, Μαρία	el
heal.committeeMemberName	Μιχαλόπουλος, Ιωάννης	el
heal.academicPublisher	Εθνικό Μετσόβιο Πολυτεχνείο. Σχολή Εφαρμοσμένων Μαθηματικών και Φυσικών Επιστημών. Τομέας Φυσικής	el
heal.academicPublisherID	ntua
heal.numberOfPages	97 σ.	el
heal.fullTextAvailability	false