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Differential gene expression in human fibroblasts simultaneously exposed to ionizing radiation and simulated microgravity

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dc.contributor.author Malatesta, Polina el
dc.contributor.author Μαλατέστα, Πολίνα en
dc.date.accessioned 2024-09-19T08:18:53Z
dc.date.available 2024-09-19T08:18:53Z
dc.identifier.uri https://dspace.lib.ntua.gr/xmlui/handle/123456789/60233
dc.identifier.uri http://dx.doi.org/10.26240/heal.ntua.27929
dc.rights Default License
dc.subject Μικροβαρύτητα el
dc.subject Διαστημική πτήση el
dc.subject Κοσμική ακτινοβολία el
dc.subject Διαφορικά Εκφρασμένα Γονίδια el
dc.subject Δίκτυα γονιδίων el
dc.subject Microgravity en
dc.subject Space flight en
dc.subject Cosmic radiation en
dc.subject Differentially Expressed Genes en
dc.subject Gene networks en
dc.title Differential gene expression in human fibroblasts simultaneously exposed to ionizing radiation and simulated microgravity en
dc.title Διαφορική γονιδιακή έκφραση σε ανθρώπινους ινοβλάστες εκτεθειμένους ταυτοχρόνων σε ιοντίζουσα ακτινοβολία και προσομοιωμένη μικροβαρύτητα el
heal.type bachelorThesis
heal.classification Βιοπληροφορική el
heal.access free
heal.recordProvider ntua el
heal.publicationDate 2024-06-25
heal.abstract During future space missions, astronauts will be exposed to cosmic radiation and microgravity (μG), known to be health risk factors. To examine the differentially expressed genes (DEGs) and their prevalent biological processes and pathways as a response to these two risk factors simultaneously, 1BR-hTERT human fibroblast cells were cultured under 1 gravity (1G) or simulated μG for 48 hrs in total and collected 0 (sham-irradiated), 3 or 24 hrs after 1 Gy of X-ray or Carbon-ion (C-ion) irradiation. A three-dimensional clinostat was used for the simulation of μG and the simultaneous radiation exposure of the samples. RNA-seq method was used to produce lists of differentially expressed genes between different environmental conditions. Over-representation analyses were performed and the enriched biological pathways and targeting transcription factors were identified. Comparing sham-irradiated cells under simulated μG and 1G conditions, terms related to response to oxygen levels and muscle contraction were identified. After irradiation with X-rays or C-ions under 1G, identified DEGs were found to be involved in DNA damage repair, signal transduction by p53 class mediator, cell cycle arrest and apoptosis pathways. The same enriched pathways emerged when cells were irradiated under simulated μG condition. Nevertheless, the combined effect attenuated the transcriptional response to irradiation which may pose a subtle risk in space flights. en
heal.advisorName Γεωργακίλας, Αλέξανδρος el
heal.committeeMemberName Γεωργακίλας, Αλέξανδρος el
heal.committeeMemberName Διακάκη, Μαρία el
heal.committeeMemberName Μιχαλόπουλος, Ιωάννης el
heal.academicPublisher Εθνικό Μετσόβιο Πολυτεχνείο. Σχολή Εφαρμοσμένων Μαθηματικών και Φυσικών Επιστημών. Τομέας Φυσικής el
heal.academicPublisherID ntua
heal.numberOfPages 97 σ. el
heal.fullTextAvailability false


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