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Effect of high-pressure treatment at various temperatures on indigenous proteolytic enzymes and whey protein denaturation in bovine milk

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dc.contributor.author Moatsou, G en
dc.contributor.author Bakopanos, C en
dc.contributor.author Katharios, D en
dc.contributor.author Katsaros, G en
dc.contributor.author Kandarakis, I en
dc.contributor.author Taoukis, P en
dc.contributor.author Politis, I en
dc.date.accessioned 2014-03-01T01:28:12Z
dc.date.available 2014-03-01T01:28:12Z
dc.date.issued 2008 en
dc.identifier.issn 0022-0299 en
dc.identifier.uri https://dspace.lib.ntua.gr/xmlui/handle/123456789/18759
dc.subject Cathepsin D en
dc.subject High pressure en
dc.subject Plasmin en
dc.subject Plasminogen activators en
dc.subject Whey proteins en
dc.subject.classification Agriculture, Dairy & Animal Science en
dc.subject.classification Food Science & Technology en
dc.subject.other cathepsin D en
dc.subject.other lactalbumin en
dc.subject.other lactoglobulin en
dc.subject.other milk protein en
dc.subject.other peptide hydrolase en
dc.subject.other plasmin en
dc.subject.other whey protein en
dc.subject.other animal en
dc.subject.other article en
dc.subject.other chemistry en
dc.subject.other enzymology en
dc.subject.other metabolism en
dc.subject.other milk en
dc.subject.other pressure en
dc.subject.other protein denaturation en
dc.subject.other temperature en
dc.subject.other Animals en
dc.subject.other Cathepsin D en
dc.subject.other Lactalbumin en
dc.subject.other Lactoglobulins en
dc.subject.other Milk en
dc.subject.other Milk Proteins en
dc.subject.other Peptide Hydrolases en
dc.subject.other Plasmin en
dc.subject.other Pressure en
dc.subject.other Protein Denaturation en
dc.subject.other Temperature en
dc.subject.other Bovinae en
dc.title Effect of high-pressure treatment at various temperatures on indigenous proteolytic enzymes and whey protein denaturation in bovine milk en
heal.type journalArticle en
heal.identifier.primary 10.1017/S002202990800321X en
heal.identifier.secondary http://dx.doi.org/10.1017/S002202990800321X en
heal.language English en
heal.publicationDate 2008 en
heal.abstract The objective of the present study was to determine the effect of high pressure (HP) processing (200, 450 and 650 MPa) at various temperatures (20, 40 and 55°C) on the total plasmin plus plasminogen-derived activity (PL), plasminogen activator(s) (PA) and cathepsin D activities and on denaturation of major whey proteins in bovine milk. Data indicated that transfer of both PL and PA from the casein micelles to milk serum occurred at all pressures utilized at room temperature (20°C). In addition to the transfer of PL and PA from micelles, there were reductions in activities of PL (16-18%) and PA (38-62%) for the pressures 450 and 650 MPa, at room temperature. There were synergistic negative effects between pressure and temperature on residual PL activity at 450 and 650 MPa and on residual PA activity only at 450 MPa. Cathepsin D activity in the acid whey from HP-treated milk was in general baroresistant at room temperature. The residual activity of cathepsin D decreased significantly at 650 MPa and 40°C and at the pressures 450 and 650 MPa at 55°C. Synergistic negative effects on the amount of native β-lactoglobulin were observed at 450 and 650 MPa and on the amount of native α-lactalbumin at 650 MPa. There were significant correlations between enzymatic activities (PL, PA and cathepsin D) and the residual native β-lactoglobulin and α-lactalbumin in bovine milk. In conclusion, HP significantly affected the activity of indigenous proteolytic enzymes and whey protein denaturation in bovine milk. Reduction in activity of indigenous enzymes (PL, PA and cathepsin D) and transfer of PL and PA from the casein to milk serum induced by HP is expected to have a profound effect on cheese yield, proteolysis during cheese ripening and quality of UHT milk during storage. © Proprietors of Journal of Dairy Research 2008. en
heal.publisher CAMBRIDGE UNIV PRESS en
heal.journalName Journal of Dairy Research en
dc.identifier.doi 10.1017/S002202990800321X en
dc.identifier.isi ISI:000258968900002 en
dc.identifier.volume 75 en
dc.identifier.issue 3 en
dc.identifier.spage 262 en
dc.identifier.epage 269 en


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