Effect of high-pressure treatment at various temperatures on indigenous proteolytic enzymes and whey protein denaturation in bovine milk

Moatsou, G; Bakopanos, C; Katharios, D; Katsaros, G; Kandarakis, I; Taoukis, P; Politis, I

dc.contributor.author	Moatsou, G	en
dc.contributor.author	Bakopanos, C	en
dc.contributor.author	Katharios, D	en
dc.contributor.author	Katsaros, G	en
dc.contributor.author	Kandarakis, I	en
dc.contributor.author	Taoukis, P	en
dc.contributor.author	Politis, I	en
dc.date.accessioned	2014-03-01T01:28:12Z
dc.date.available	2014-03-01T01:28:12Z
dc.date.issued	2008	en
dc.identifier.issn	0022-0299	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/18759
dc.subject	Cathepsin D	en
dc.subject	High pressure	en
dc.subject	Plasmin	en
dc.subject	Plasminogen activators	en
dc.subject	Whey proteins	en
dc.subject.classification	Agriculture, Dairy & Animal Science	en
dc.subject.classification	Food Science & Technology	en
dc.subject.other	cathepsin D	en
dc.subject.other	lactalbumin	en
dc.subject.other	lactoglobulin	en
dc.subject.other	milk protein	en
dc.subject.other	peptide hydrolase	en
dc.subject.other	plasmin	en
dc.subject.other	whey protein	en
dc.subject.other	animal	en
dc.subject.other	article	en
dc.subject.other	chemistry	en
dc.subject.other	enzymology	en
dc.subject.other	metabolism	en
dc.subject.other	milk	en
dc.subject.other	pressure	en
dc.subject.other	protein denaturation	en
dc.subject.other	temperature	en
dc.subject.other	Animals	en
dc.subject.other	Cathepsin D	en
dc.subject.other	Lactalbumin	en
dc.subject.other	Lactoglobulins	en
dc.subject.other	Milk	en
dc.subject.other	Milk Proteins	en
dc.subject.other	Peptide Hydrolases	en
dc.subject.other	Plasmin	en
dc.subject.other	Pressure	en
dc.subject.other	Protein Denaturation	en
dc.subject.other	Temperature	en
dc.subject.other	Bovinae	en
dc.title	Effect of high-pressure treatment at various temperatures on indigenous proteolytic enzymes and whey protein denaturation in bovine milk	en
heal.type	journalArticle	en
heal.identifier.primary	10.1017/S002202990800321X	en
heal.identifier.secondary	http://dx.doi.org/10.1017/S002202990800321X	en
heal.language	English	en
heal.publicationDate	2008	en
heal.abstract	The objective of the present study was to determine the effect of high pressure (HP) processing (200, 450 and 650 MPa) at various temperatures (20, 40 and 55°C) on the total plasmin plus plasminogen-derived activity (PL), plasminogen activator(s) (PA) and cathepsin D activities and on denaturation of major whey proteins in bovine milk. Data indicated that transfer of both PL and PA from the casein micelles to milk serum occurred at all pressures utilized at room temperature (20°C). In addition to the transfer of PL and PA from micelles, there were reductions in activities of PL (16-18%) and PA (38-62%) for the pressures 450 and 650 MPa, at room temperature. There were synergistic negative effects between pressure and temperature on residual PL activity at 450 and 650 MPa and on residual PA activity only at 450 MPa. Cathepsin D activity in the acid whey from HP-treated milk was in general baroresistant at room temperature. The residual activity of cathepsin D decreased significantly at 650 MPa and 40°C and at the pressures 450 and 650 MPa at 55°C. Synergistic negative effects on the amount of native β-lactoglobulin were observed at 450 and 650 MPa and on the amount of native α-lactalbumin at 650 MPa. There were significant correlations between enzymatic activities (PL, PA and cathepsin D) and the residual native β-lactoglobulin and α-lactalbumin in bovine milk. In conclusion, HP significantly affected the activity of indigenous proteolytic enzymes and whey protein denaturation in bovine milk. Reduction in activity of indigenous enzymes (PL, PA and cathepsin D) and transfer of PL and PA from the casein to milk serum induced by HP is expected to have a profound effect on cheese yield, proteolysis during cheese ripening and quality of UHT milk during storage. © Proprietors of Journal of Dairy Research 2008.	en
heal.publisher	CAMBRIDGE UNIV PRESS	en
heal.journalName	Journal of Dairy Research	en
dc.identifier.doi	10.1017/S002202990800321X	en
dc.identifier.isi	ISI:000258968900002	en
dc.identifier.volume	75	en
dc.identifier.issue	3	en
dc.identifier.spage	262	en
dc.identifier.epage	269	en