Inactivation kinetics of peach pulp pectin methylesterase as a function of high hydrostatic pressure and temperature process conditions

Boulekou, SS; Katsaros, GJ; Taoukis, PS

dc.contributor.author	Boulekou, SS	en
dc.contributor.author	Katsaros, GJ	en
dc.contributor.author	Taoukis, PS	en
dc.date.accessioned	2014-03-01T01:33:38Z
dc.date.available	2014-03-01T01:33:38Z
dc.date.issued	2010	en
dc.identifier.issn	1935-5130	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/20503
dc.subject	Arrhenius	en
dc.subject	Enzyme kinetics	en
dc.subject	High pressure	en
dc.subject	Peach	en
dc.subject	Pectin methylesterase	en
dc.subject	Thermal inactivation	en
dc.subject.other	Activation volume	en
dc.subject.other	Ambient pressures	en
dc.subject.other	Antagonistic effects	en
dc.subject.other	Arrhenius	en
dc.subject.other	First order kinetics	en
dc.subject.other	Good correlations	en
dc.subject.other	High hydrostatic pressure	en
dc.subject.other	High pressure	en
dc.subject.other	High temperature	en
dc.subject.other	Inactivation kinetics	en
dc.subject.other	Kinetic study	en
dc.subject.other	Modeling approach	en
dc.subject.other	Moderate temperature	en
dc.subject.other	Multi-parameter models	en
dc.subject.other	Peach pulp	en
dc.subject.other	Pectin methylesterase	en
dc.subject.other	Pressure and temperature	en
dc.subject.other	Pressure ranges	en
dc.subject.other	Process condition	en
dc.subject.other	Quantitative estimation	en
dc.subject.other	Temperature conditions	en
dc.subject.other	Thermal inactivation	en
dc.subject.other	Activation energy	en
dc.subject.other	Enzyme kinetics	en
dc.subject.other	Enzymes	en
dc.subject.other	Hydraulics	en
dc.subject.other	Hydrodynamics	en
dc.subject.other	Hydrostatic pressure	en
dc.subject.other	Rate constants	en
dc.subject.other	Pressure effects	en
dc.subject.other	Prunus persica	en
dc.title	Inactivation kinetics of peach pulp pectin methylesterase as a function of high hydrostatic pressure and temperature process conditions	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s11947-008-0132-4	en
heal.identifier.secondary	http://dx.doi.org/10.1007/s11947-008-0132-4	en
heal.language	English	en
heal.publicationDate	2010	en
heal.abstract	A kinetic study of the inactivation of endogenous pectin methylesterase (PME) in Greek commercial peach pulp under high hydrostatic pressure (HHP; 100-800 MPa) combined with moderate temperature (30-70 °C) was conducted. Thermal inactivation of the enzyme at ambient pressure conditions was also studied. PME inactivation was modeled by first order kinetics at all conditions tested. High pressure and temperature acted synergistically on PME inactivation, except at the high temperature of 70 °C at the middle pressure range (100-600 MPa), where an antagonistic effect of pressure and temperature was observed. At this specific middle pressure range, an increase of pressure processing led to increased inactivation rate constants of peach PME. A multiparameter model was developed to express the PME inactivation rate constant as a function of temperature and pressure process conditions, taking into account the dependence of both activation energy and activation volume on pressure and temperature, respectively. A good correlation between experimental and predicted values of inactivation rate constants was established. This modeling approach enables the quantitative estimation of the HHP-temperature conditions needed to achieve a targeted PME inactivation in the peach pulp. © 2008 Springer Science + Business Media, LLC.	en
heal.publisher	SPRINGER	en
heal.journalName	Food and Bioprocess Technology	en
dc.identifier.doi	10.1007/s11947-008-0132-4	en
dc.identifier.isi	ISI:000280596600008	en
dc.identifier.volume	3	en
dc.identifier.issue	5	en
dc.identifier.spage	699	en
dc.identifier.epage	706	en