Immunodetection and clinico-pathologlical correlates of two tumour growth regulators in laryngeal carcinoma

Lazaris, AC; Rigopoulou, A; Tseleni-Balafouta, S; Kavantzas, N; Thimara, I; Zorzos, HS; Eutychiadis, CA; Petraki, K; Kandiloros, D; Davaris, P

dc.contributor.author	Lazaris, AC	en
dc.contributor.author	Rigopoulou, A	en
dc.contributor.author	Tseleni-Balafouta, S	en
dc.contributor.author	Kavantzas, N	en
dc.contributor.author	Thimara, I	en
dc.contributor.author	Zorzos, HS	en
dc.contributor.author	Eutychiadis, CA	en
dc.contributor.author	Petraki, K	en
dc.contributor.author	Kandiloros, D	en
dc.contributor.author	Davaris, P	en
dc.date.accessioned	2014-03-01T01:52:05Z
dc.date.available	2014-03-01T01:52:05Z
dc.date.issued	2002	en
dc.identifier.issn	0213-3911	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/26559
dc.subject	larynx	en
dc.subject	squamous cell carcinoma	en
dc.subject	Ki67	en
dc.subject	TRF1	en
dc.subject	immunohistochemistry	en
dc.subject.classification	Cell Biology	en
dc.subject.classification	Pathology	en
dc.subject.other	SQUAMOUS-CELL CARCINOMA	en
dc.subject.other	HUMAN TELOMERIC PROTEIN	en
dc.subject.other	PROGNOSTIC FACTORS	en
dc.subject.other	KI-67 ANTIGEN	en
dc.subject.other	CANCER	en
dc.subject.other	TRF1	en
dc.subject.other	LESIONS	en
dc.subject.other	GRADE	en
dc.subject.other	P53	en
dc.title	Immunodetection and clinico-pathologlical correlates of two tumour growth regulators in laryngeal carcinoma	en
heal.type	journalArticle	en
heal.language	English	en
heal.publicationDate	2002	en
heal.abstract	Activation of telomerase, present in the vast majority of all human cancers, is associated with elongation of chromosomal telomeres and consequent cell immortalization. Telomere length homeostasis is a dynamic process governed by the negative feedback mechanism of the telomeric repeat binding factor I (TRF1) which inhibits the action of telomerase in telomerase-positive cells. In an attempt to investigate markers of tumour growth as possible prognostic indicators in laryngeal cancer, we studied the expression of TRF1 and of the proliferation marker Ki67 on 96 invasive squamous carcinomas of the larynx. A standard three step immunoperoxidase staining method was applied on paraffin sections incubated with appropriate polyclonal antibodies. The percentages of Ki67- and TRF1-immunopositive cancerous cells were calculated by image analysis. Univariate and multivariate statistical analysis of the staining results were performed in order to detect any association of the examined immunomarkers with the tumours' classical clinicopathological variables including nuclear morphometric features as well as with patients' disease-free survival. Ki67 immunostaining was positively linked with advanced patients' age, nodal involvement as well as presence of early recurrence. No relation was found between proliferative fraction and TRF1 immunoexpression. TRF1 was expressed in 55.2% of all cases and was positively linked only to tumour size. Multivariate statistical analysis revealed the presence of lymph nodal metastasis and Ki67 immunopositivity index greater than or equal to 20% as significant predictors of relapse. Increased Ki67 immunostaining appears to be a promising marker of tumour aggressiveness in laryngeal cancer. After one point at the tumour's natural history, the maintenance of tumour growth does not seem to depend on cell proliferation but on TRF1 immunoexpression. Whether the latter can be used for the identification of immortalized cells in every-day practice is worth investigating.	en
heal.publisher	F HERNANDEZ	en
heal.journalName	HISTOLOGY AND HISTOPATHOLOGY	en
dc.identifier.isi	ISI:000173327500014	en
dc.identifier.volume	17	en
dc.identifier.issue	1	en
dc.identifier.spage	131	en
dc.identifier.epage	138	en