A photosynthetic biosensor with enhanced electron transfer generation realized by laser printing technology

Touloupakis, E; Boutopoulos, C; Buonasera, K; Zergioti, I; Giardi, MT

dc.contributor.author	Touloupakis, E	en
dc.contributor.author	Boutopoulos, C	en
dc.contributor.author	Buonasera, K	en
dc.contributor.author	Zergioti, I	en
dc.contributor.author	Giardi, MT	en
dc.date.accessioned	2014-03-01T02:07:23Z
dc.date.available	2014-03-01T02:07:23Z
dc.date.issued	2012	en
dc.identifier.issn	16182642	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/29558
dc.subject	Biosensor	en
dc.subject	Herbicides	en
dc.subject	Laser printing	en
dc.subject	Photosynthesis	en
dc.subject.other	Current intensity	en
dc.subject.other	Electrochemical biosensor	en
dc.subject.other	Electron transfer	en
dc.subject.other	Energy productions	en
dc.subject.other	Laser printing	en
dc.subject.other	Laser-induced forward transfer	en
dc.subject.other	Nanomolar concentration	en
dc.subject.other	Photocurrent signals	en
dc.subject.other	Photosynthetic systems	en
dc.subject.other	Response signal	en
dc.subject.other	Screen printed electrodes	en
dc.subject.other	Thylakoids	en
dc.subject.other	Biological materials	en
dc.subject.other	Biomimetics	en
dc.subject.other	Electron transitions	en
dc.subject.other	Herbicides	en
dc.subject.other	Photosynthesis	en
dc.subject.other	Printing	en
dc.subject.other	Printing presses	en
dc.subject.other	Weed control	en
dc.subject.other	Biosensors	en
dc.subject.other	herbicide	en
dc.subject.other	plant extract	en
dc.subject.other	article	en
dc.subject.other	chemistry	en
dc.subject.other	electrode	en
dc.subject.other	genetic procedures	en
dc.subject.other	instrumentation	en
dc.subject.other	methodology	en
dc.subject.other	photosynthesis	en
dc.subject.other	printing	en
dc.subject.other	spinach	en
dc.subject.other	thylakoid	en
dc.subject.other	Biosensing Techniques	en
dc.subject.other	Electrodes	en
dc.subject.other	Herbicides	en
dc.subject.other	Photosynthesis	en
dc.subject.other	Plant Extracts	en
dc.subject.other	Printing	en
dc.subject.other	Spinacia oleracea	en
dc.subject.other	Thylakoids	en
dc.title	A photosynthetic biosensor with enhanced electron transfer generation realized by laser printing technology	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s00216-012-5771-7	en
heal.identifier.secondary	http://dx.doi.org/10.1007/s00216-012-5771-7	en
heal.publicationDate	2012	en
heal.abstract	One of the limits of current electrochemical biosensors is a lack of methods providing stable and highly efficient junctions between biomaterial and solid-state devices. This paper shows how laser-induced forward transfer (LIFT) can enable efficient electron transfer from photosynthetic biomaterial immobilized on screen-printed electrodes (SPE). The ideal pattern, in terms of photocurrent signal of thylakoid droplets giving a stable response signal with a current intensity of approximately 335∈±∈13 nA for a thylakoid mass of 28∈±∈4 ng, was selected. It is shown that the efficiency of energy production of a photosynthetic system can be strongly enhanced by the LIFT process, as demonstrated by use of the technique to construct an efficient and sensitive photosynthesis-based biosensor for detecting herbicides at nanomolar concentrations. © 2012 Springer-Verlag.	en
heal.journalName	Analytical and Bioanalytical Chemistry	en
dc.identifier.doi	10.1007/s00216-012-5771-7	en
dc.identifier.volume	402	en
dc.identifier.issue	10	en
dc.identifier.spage	3237	en
dc.identifier.epage	3244	en