Human fibroblast alterations induced by low power laser irradiation at the single cell level using confocal microscopy

Alexandratou, E; Yova, D; Handris, P; Kletsas, D; Loukas, S

dc.contributor.author	Alexandratou, E	en
dc.contributor.author	Yova, D	en
dc.contributor.author	Handris, P	en
dc.contributor.author	Kletsas, D	en
dc.contributor.author	Loukas, S	en
dc.date.accessioned	2014-03-01T11:44:30Z
dc.date.available	2014-03-01T11:44:30Z
dc.date.issued	2002	en
dc.identifier.issn	1474-905X	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/36988
dc.subject	Confocal Microscopy	en
dc.subject	Low Power	en
dc.subject	Single Cell	en
dc.subject.classification	Biochemistry & Molecular Biology	en
dc.subject.classification	Biophysics	en
dc.subject.classification	Chemistry, Physical	en
dc.subject.other	calcium ion	en
dc.subject.other	fluorescent dye	en
dc.subject.other	reactive oxygen metabolite	en
dc.subject.other	alkalinization	en
dc.subject.other	calcium cell level	en
dc.subject.other	cell activity	en
dc.subject.other	cell culture	en
dc.subject.other	cell membrane potential	en
dc.subject.other	cell pH	en
dc.subject.other	cell proliferation	en
dc.subject.other	cell stimulation	en
dc.subject.other	computer program	en
dc.subject.other	confocal laser microscopy	en
dc.subject.other	controlled study	en
dc.subject.other	cytosol	en
dc.subject.other	fibroblast	en
dc.subject.other	human	en
dc.subject.other	human cell	en
dc.subject.other	image analysis	en
dc.subject.other	image processing	en
dc.subject.other	in vivo study	en
dc.subject.other	incubation time	en
dc.subject.other	low level laser therapy	en
dc.subject.other	mitochondrial membrane	en
dc.subject.other	priority journal	en
dc.subject.other	quantitative analysis	en
dc.subject.other	regulatory mechanism	en
dc.subject.other	review	en
dc.subject.other	signal processing	en
dc.subject.other	spike	en
dc.subject.other	article	en
dc.subject.other	confocal microscopy	en
dc.subject.other	intracellular membrane	en
dc.subject.other	laser	en
dc.subject.other	metabolism	en
dc.subject.other	pH	en
dc.subject.other	physiology	en
dc.subject.other	radiation exposure	en
dc.subject.other	radiation response	en
dc.subject.other	Cells, Cultured	en
dc.subject.other	Dose-Response Relationship, Radiation	en
dc.subject.other	Fibroblasts	en
dc.subject.other	Humans	en
dc.subject.other	Hydrogen-Ion Concentration	en
dc.subject.other	Intracellular Membranes	en
dc.subject.other	Lasers	en
dc.subject.other	Membrane Potentials	en
dc.subject.other	Microscopy, Confocal	en
dc.subject.other	Reactive Oxygen Species	en
dc.title	Human fibroblast alterations induced by low power laser irradiation at the single cell level using confocal microscopy	en
heal.type	other	en
heal.identifier.primary	10.1039/b110213n	en
heal.identifier.secondary	http://dx.doi.org/10.1039/b110213n	en
heal.language	English	en
heal.publicationDate	2002	en
heal.abstract	Low power laser irradiation is regarded to have a significant role in triggering cellular proliferation and in treating diseases of diverse etiologies. The present work contributes to the understanding of the mechanisms of action by studying low power laser effects in human fibroblasts. Confocal laser scanning microscopy is used for irradiation and observation of the same area of interest allowing the imaging of laser effects at the single cell level and in real time. Coverslip cultures were placed in a small incubation chamber for in vivo microscopic observation. Laser stimulation of the cells was performed using the 647 nm line of the confocal laser through the objective lens of the microscope. Mitochondrial membrane potential (DeltaPsi(m)), intracellular pH, calcium alterations and generation of reactive oxygen species (ROS) were monitored using specific fluorescent vital probes. The induced effects were quantified using digital image processing techniques. After laser irradiation, a gradual alkalinization of the cytosolic pH and an increase in mitochondrial membrane potential were observed. Recurrent spikes of intracellular calcium concentration were also triggered by laser. Reactive oxygen species were generated as a result of biostimulation. No such effects were monitored in microscopic fields other than the irradiated ones.	en
heal.publisher	ROYAL SOC CHEMISTRY	en
heal.journalName	Photochemical and Photobiological Sciences	en
dc.identifier.doi	10.1039/b110213n	en
dc.identifier.isi	ISI:000177773500002	en
dc.identifier.volume	1	en
dc.identifier.issue	8	en
dc.identifier.spage	547	en
dc.identifier.epage	552	en